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A practice-oriented guide to assaying more than 100 of the most important enzymes, complete with the theoretical background and specific protocols for immediate use in the biochemical laboratory. Now expanded with a new section on metal ion determination.
MOLECULAR ENZYMOLOGY, BECAUSE OF ITS CHEMICAL AND MATHEMATICAL content, is often regarded as a formidable and forbidding topic by undergraduates on a biology or biochemistry course, As a result of teaching enzymology to undergraduates for a number of years, we recognize the areas which appear to cause the most common difficulties in conceptual understanding. We feel that a book treating those areas by means of a logical approach carefully developed from basic principles fills a gap in the multiplicity of enzymology texts currently available. In writing this book we h;:lVe had in mind the needs of Honours Biochemistry students, in particular those who may take a special interest in enzymology...
This enzymology textbook for graduate and advanced undergraduate students covers the syllabi of most universities where this subject is regularly taught. It focuses on the synchrony between the two broad mechanistic facets of enzymology: the chemical and the kinetic, and also highlights the synergy between enzyme structure and mechanism. Designed for self-study, it explains how to plan enzyme experiments and subsequently analyze the data collected. The book is divided into five major sections: 1] Introduction to enzymes, 2] Practical aspects, 3] Kinetic Mechanisms, 4] Chemical Mechanisms, and 5] Enzymology Frontiers. Individual concepts are treated as stand-alone chapters; readers can explor...
Provided in this book are guidelines and practical advice for anyone working with enzymes.
Enzymology is designed as a full-fledge textbook for the undergraduate engineering students of Biotechnology and Chemical Engineering. In addition, this book would also serve as an invaluable reference for students who are pursuing their graduate and postgraduate degree programs in Biotechnology, and all other life sciences programs that offer a course on Enzymes. The book covers all the fundamental and inevitable concepts like Enzyme Kinetics, Enzyme Inhibition, Enzyme Activity Regulation and proceeds in to discussion of applications of enzymes in various domains including Molecular Biology, Cloning and Genetic Engineering. A separate chapter has been devoted to the study of Enzyme Engineering and Technology, which the engineering students would find useful. Comprehensive in its coverage of topics, the book is rich in features like illustrations supporting the theoretical discussion, chapter-end summary, glossary of important terms and review questions to reinforce the learning. Numerical problems too have been provided in all the relevant chapters.
Protocols and Applications in Enzymology provides instruction on the experimental procedures of enzyme isolation techniques, innovative screening techniques, and instrument enabled enzyme assays and their underlying principles, among other protocols. The book serves as a one-stop solution for those working with different enzyme protocols in the fields of biochemistry, microbiology, biotechnology and allied subjects. Each chapter offers a full overview of protocol key resources, materials required, quantifiable and statistical analysis, optimization and troubleshooting, safety considerations, and standards. Applications are discussed across distribution and diversity of microbial enzymes, enz...
Exceptionally clear coverage of mechanisms for catalysis, forces in aqueous solution, carbonyl- and acyl-group reactions, practical kinetics, more.
This thoroughly revised edition successful and popular Fundamentals of Enzymology emphasizes the new advances in enzymology research while consolidating the strengths of the previous new editions. It is excellent for advance undergraduates in biochemistry and molecular biology.
Soil enzymes are one of the vital key mediators involved in nutrient recycling and the decomposition of organic matter and thereby in maintaining soil quality and fertility. This Soil Biology volume covers the various facets of soil enzymes, such as their functions, biochemical and microbiological properties and the factors affecting their activities. Enzymes in the rhizosphere, in forest soils, and in volcanic ash-derived soils are described. Soil enzymes covered include phosphohydrolases, lignocellulose-degrading enzymes, phenol oxidases, fungal oxidoreductases, keratinases, pectinases, xylanases, lipases and pectinases. Several chapters treat the soil enzymatic activities in the bioremediation of soils contaminated with pesticides and pollutants such as oil, chlorinated compounds, synthetic dyes and aromatic hydrocarbons. The role of soil enzymes as bioindicators is a further important topic addressed.
De Novo Enzyme Design, the newest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume includes the design of metal binding maquettes, insertion of non-natural cofactors, Cu metallopeptides, non-covalent interactions in peptide assemblies, peptide binding and bundling, heteronuclear metalloenzymes, florinated peptides, De Novo imaging agents, and protein-protein interaction. - Continues the legacy of this premier serial with quality chapters on de novo enzyme design - Represents the newest volume in the Methods in Enzymology series, providing premier, quality chapters authored by leaders in the field - Ideal reference for those interested in the study of enzyme design that looks at both structure and mechanism