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Biological and chemical sciences have undergone an unprecedented transformation, reflected by the huge use of parallel and automated technologies in key fields such as genome sequencing, DNA chips, nanoscale functional biology or combinatorial chemistry. It is now possible to generate and store from tens of thousands to millions of new small molecules, based on enhanced chemical synthesis strategies. Automated screening of small molecules is one of the technologies that has revolutionized biology, first developed for the pharmaceutical industry and recently introduced in academic laboratories. High-throughput and high-content screening allow the identification of bioactive compounds in colle...
This second edition volume expands on the previous edition with new and updated chapters that explore our current view on plastid evolution, structure, and function. The chapters in this book are organized into three parts and cover topics such as plastid evolution, plasticity, and functional and structural diversity; techniques used to visualize, fractionate, purify, and study primary plastids from plant materials, and secondary plastids; and methods to analyze plastids by integrated biology strategies based on genetics, genomics, proteomics, and lipidomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Plastids: Methods and Protocols, Second Edition is a valuable resource that will help students, engineers, and researchers further explore and understand this fascinating organelle. .
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This second edition volume expands on the previous edition with new and updated chapters that explore our current view on plastid evolution, structure, and function. The chapters in this book are organized into three parts and cover topics such as plastid evolution, plasticity, and functional and structural diversity; techniques used to visualize, fractionate, purify, and study primary plastids from plant materials, and secondary plastids; and methods to analyze plastids by integrated biology strategies based on genetics, genomics, proteomics, and lipidomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Plastids: Methods and Protocols, Second Edition is a valuable resource that will help students, engineers, and researchers further explore and understand this fascinating organelle. .
A collection of papers that comprehensively describe the major areas of research on lipid metabolism of plants. State-of-the-art knowledge about research on fatty acid and glycerolipid biosynthesis, isoprenoid metabolism, membrane structure and organization, lipid oxidation and degradation, lipids as intracellular and extracellular messengers, lipids and environment, oil seeds and gene technology is reviewed. The different topics covered show that modern tools of plant cellular and molecular biology, as well as molecular genetics, have been recently used to characterize several key enzymes of plant lipid metabolism (in particular, desaturases, thioesterases, fatty acid synthetase) and to iso...
Lipids in Photosynthesis: Essential and Regulatory Functions, provides an essential summary of an exciting decade of research on relationships between lipids and photosynthesis. The book brings together extensively cross-referenced and peer-reviewed chapters by prominent researchers. The topics covered include the structure, molecular organization and biosynthesis of fatty acids, glycerolipids and nonglycerolipids in plants, algae, lichens, mosses, and cyanobacteria, as well as in chloroplasts and mitochondria. Several chapters deal with the manipulation of the extent of unsaturation of fatty acids and the effects of such manipulation on photosynthesis and responses to various forms of stress. The final chapters focus on lipid trafficking, signaling and advanced analytical techniques. Ten years ago, Siegenthaler and Murata edited "Lipids in Photosynthesis: Structure, Function and Genetics," which became a classic in the field. "Lipids in Photosynthesis: Essential and Regulatory Functions," belongs, with its predecessor, in every plant and microbiological researcher's bookcase.
This book covers the expression of photosynthesis related genes including regulation both at transcriptional and translational levels. It reviews biogenesis, turnover, and senescence of thylakoid pigment protein complexes and highlights some crucial regulatory steps in carbon metabolism.