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Non-Conventional Yeasts in Genetics, Biochemistry and Biotechnology
Most information on yeasts derives from experiments with the conventional yeasts Saccaromyces cerevisiae and Schizossaccharomyces pombe, the complete nuclear and mitochondrial genome of which has also been sequenced. For all other non-conventional yeasts, investigations are in progress and the rapid development of molecular techniques has allowed an insight also into a variety of non-conventional yeasts. In this bench manual, over 70 practical protocols using 15 different non-conventional yeast species and in addition several protocols of general use are described in detail. All of these experiments on the genetics, biochemistry and biotechnology of yeasts have been contributed by renowned laboratories and have been reproduced many times. The reliable protocols are thus ideally suited also for undergraduate and graduate practical courses.
Yarrowia lipolytica
Due to various special physiological features and a genome that greatly differs in structure, gene content and organization from other yeasts, Y. lipolytica is widely used as a host system. With its characteristics, such as the ability to grow on lipids or grease, to accumulate oil and the high capacity for secretion of proteases and lipases, the yeast is of great interest for biotechnological applications. The main topics covered in this Microbiology Monograph are: expression and secretion of heterologous proteins; acid and alkaline extracellular proteases; genetics, production, biochemical characterization and biotechnological application of lipases; production and secretion of several organic acids and flagrances; as well as the functional expression of P450 systems and its use in steroid biotransformation.
Yarrowia lipolytica
Due to various special physiological features and a genome that greatly differs in structure, gene content and organization from other yeasts, Y. lipolytica is widely used as a model organism. With its characteristics, such as the ability to accumulate oil and the high capacity for secretion of proteases and lipases, the yeast is also of great interest for biotechnological applications. The main topics covered in this Microbiology Monograph are: comparative genomics; mitochondrial genomics and proteomics, including the analysis of the respiratory chain; transposable elements and their activities; non-coding RNA genes, which display a number of unusual and remarkable features compared to other hemiascomycetes; utilization of hydrophobic substrates, of n-alkane and its oxidized derivatives as sources of carbon and energy; ambient pH signalling; comparison of protein families in non-conventional yeasts and S. cerevisiae; and the sulphur metabolism of cheese-ripening yeast. .
Subsampling GPS Receiver Front-end
Research in RFIC design has recently shifted towards direct conversion and subsampling architectures as an alternative to the conventional super-heterodyne architectures. Bandpass sampling architectures, also called subsampling architectures, exhibit several advantages over super-heterodyne architectures, notably, the complexity of subsampling architectures is significantly lower since no phase-locked loop is required. A direct consequence is that downconversion from RF to IF can be achieved with significant power savings as compared to the super-heterodyne architecture. Another significant benefit of such architectures is the capability for processing multiple signals in parallel. The abili...
Reprogramming Microbial Metabolic Pathways
Metabolic engineering has been developed over the past 20 years to become an important tool for rational engineering of microorganisms. This book has a particular interest in the methods and applications of metabolic engineering to improve the production and yield of a variety of metabolites in microorganisms. The overall goal is to achieve a better understanding of metabolism in different microorganisms, and provide a rational basis to reprogram microorganisms for improved biochemical production. This book brings together contributions from leading researchers at the cutting edge of these topics. The subject matter is divided into two sections. The first section deals with novel and emerging methods for redesigning microorganisms exploiting systems biology and gene regulation. The second discusses practical aspects of metabolic engineering for over production of a variety of valuable chemicals and materials by fermentation.
Appreciating Local Knowledge
In the light of the globalization, (post-)modernization, social fragmentation, and economization of many of today’s living contexts, local knowledge is receiving increasing attention in various sciences. Commonly, local knowledge indicates a counterpart to both rational forms of an explicit knowledge of facts and knowledge of universal validity. Local knowledge attempts to appreciate a more comprehensive view of people’s skills, capabilities, experience, and sophistication. On the other hand, the reference to ‘local’ implies an idea of bounded applicability of knowledge in a specific environment. Beyond this scope of application, local knowledge can be acknowledged either as instrume...
The Object and Purpose of Intellectual Property
Much of the debate around the parameters of intellectual property (IP) protection relates to differing views about what IP law is supposed to achieve. This book analyses the object and purpose of international intellectual property law, examining how international agreements have been interpreted in different jurisdictions and how this has led to diversity in IP regimes at a national level.
Functional Genetics of Industrial Yeasts
In recent years, new yeast species have proven their value and novel biotechnological applications have emerged. This book compiles the multi-faceted genetic repertoire of several yeasts relevant to modern biotechnology, and describes their utilization in research and application in the light of their genetic make-up and physiological characteristics. Moreover, the book presents a thorough overview of a wide array of methodologies from classical genetics to modern genomics technologies that have been and are being used in functional analysis of yeasts.
