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Biotic stress can increase or reduce a plant's susceptibility to specific pathogens. A wide range of plant pathogens, i.e., viruses, bacteria, and fungi causing diseases, modulate different elements of plant defense mechanisms responses that influence disease and the physiological state of host plants. Nevertheless, plant hosts are not static and defenseless. Plants have developed active and dynamic complex defense mechanisms to protect themselves against different pathogenic stressors. Plants' defense mechanisms consist of multi-elements and can be determined by pre-formed, natural barriers or inducible defense responses directly activated upon detection of a pathogen. The inducible respons...
Plant pathogens cause significant economic losses and endanger agricultural sustainability. The emergence of new plant diseases is caused primarily by international trade, climate change, and pathogens' ability to evolve quickly. Rapid and accurate identification of plant pathogens is critical for disease management. The diversity and distribution of plant pathogens, on the other hand, can significantly impede disease management and diagnostic efforts. Plant pathogens employ a number of strategies that result in diversity, transmission, and host adaptation. Plant pathogens have been observed interacting with a wide range of host species such as plants, endophytes, insects, pollinators, and other plant pathogens. However, the transmission and evolution of plant pathogens in hosts, as well as the impact of pathogens on different hosts, are often unknown.
The aim of Plant Virology Protocols is to provide a source of infor- tion to guide the reader through the wide range of methods involved in gen- ating transgenic plants that are resistant to plant viruses. To this end, we have commissioned a wide-ranging list of chapters that will cover the methods required for: plant virus isolation; RNA extraction; cloning coat p- tein genes; introduction of the coat protein gene into the plant genome; and testing transgenic plants for resistance. The book then moves on to treatments of the mechanisms of resistance, the problems encountered with field testing, and key ethical issues surrounding transgenic technology. Although Plant Virology Protocols deals with the cloning and expression of the coat protein gene, the techniques described can be equally applied to other viral genes and nucleotide sequences, many of which have also been shown to afford protection when introduced into plants. The coat protein has, however, been the most widely applied, and as such has been selected to illustrate the techniques involved. Plant Virology Protocols has been divided into six major sections, c- taining 55 chapters in total.
Plant RNA Viruses: Molecular Pathogenesis and Management provides wide-ranging coverage on the recognition and signaling events between plants and RNA viruses. The book examines the molecular biology of signaling, host-virus interaction, RNA virus diversity, and how plants and cellular pathogens interact. Sections cover Virus Diversity and Diagnosis, Virus-Host Interactions and Virus Management. Specific chapters discuss classification and nomenclature of viruses, detail the molecular characteristics of viral genomes, highlight the viral manipulation of cellular key regulatory systems for successful virus infection, and discuss the movement of plant viruses into plant cells. Additional topic...
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