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Bioprocessing of Viral Vaccines
This book focuses on cell culture-produced viral vaccines to meet the needs of the rapidly expanding research and development in academia and industry in the field. This book introduces the basic principles of vaccination and the manufacturing of viral vaccines. Bioprocessing of Viral Vaccines, will provide an overview of the advanced strategies needed to respond to the challenges of new and established viral infection diseases. The first few chapters cover the basics of virology and immunology as essential concepts to understand the function and design of viral vaccines. The core of the content is dedicated to process development, including upstream processing and cell culture of viral vacc...
Assessing the Role of Copy Number Variations, Mitochondrial DNA and MicroRNAs in Neurodegenerative Disorders
Les malalties neurodegeneratives són patologies complexes i progressives que afecten milions de persones a tot el món. Entre d'altres, la malaltia d'Alzheimer (MA), la malaltia de Parkinson (MP) i la demència frontotemporal (DFT) són les tres condicions més prevalents. Tot i l'extensiva recerca duta a terme, els esdeveniments moleculars que desencadenen aquestes patologies són encara desconeguts.L'objectiu d'aquesta tesi és entendre el rol de determinats factors genètics i epigenètics en les malalties neurodegeneratives, a través de l'estudi de les reestructuracions genètiques, i la quantificació d'ADN mitocondrial circulant i espècies d'ARN no codificants.Primerament, s'ha anal...
Strategies for Improving Production Levels of HIV-1 VLPs by Transient Transfection of HEK 293 Suspension Cultures
Les partícules similars a virus (VLP) ofereixen un gran potencial com a candidates per a la nova producció de vacunes. En aquest treball es presenta el desenvolupament i l'optimització d'un protocol de producció de VLP de Gag d'VIH-1 mitjançant l'expressió gènica transitòria en cultius de cèl·lules HEK 293 en suspensió. La transfecció transitòria permet una generació ràpida de proteïnes recombinants en quantitat i qualitat suficient per realitzar assajos preclínics, i és particularment interessant en les fases primerenques del desenvolupament. Aquest treball es divideix en quatre capítols principals. Al primer capítol, el medi comercial lliure de sèrum Freestyle 293 s'op...
Salvador Dali
Salvador Dalí: mad genius or cunning manipulator? That question has haunted Dalí's career. Ever since he burst upon the scene in the 1920s with his astounding draughtsmanship and surrealistic vision he has been both admired and reviled. Meryle Secrest goes behind the carefully maintained façade to reveal many hitherto unknown details of Dalí's troubled childhood, suggesting that the artist's early works are actually autobiographical to a much greater extent than has been thought. Her study examines Dalí's childhood to find the origins of his later behavior and the reason for his frantic attempts to assert his individuality. Dalí's emotional crises, his successes and failures in Europe and America, his careers as artist, designer and showman, are vividly and compelling described, as is his mysterious near-death in a 1984 fire and his final years as prisoner of his own self-made persona. -- From publisher's description.
Owl Monkeys
This book integrates three decades of owl monkey research conducted since 1994 when the first and only book focused on the genus Aotus was published. Owl monkeys were one of the least understood primates then; knowledge from wild populations was only beginning to emerge and there had been some substantial research in colonies of captive individuals. The situation is very different today. Research on captive owl monkeys has continued to develop, with valuable contributions to the health and medical sciences. And there is now enough information on the behavior, ecology, conservation, and biogeography of the genus that merits a synthesis. The book synthesizes new field data on the biogeography,...
La nube gris
La nube gris cuenta la historia de una niña muy enfadada que viaja por el mundo buscando la manera de deshacerse de su enfado. En su camino encontrará varios personajes que con sus consejos la ayudarán a eliminarlo. Una bonita historia para niños de 2 a 4 años que ayudará en la gestión de las rabietas propias de la edad. Un cuento interactivo para los más pequeños que propone de recursos para afrontar el enfado.
A Laura, potser
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