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This unique text/reference presents an overview of the computational aspects of protein crystallization, describing how to build robotic high-throughput and crystallization analysis systems. The coverage encompasses the complete data analysis cycle, including the set-up of screens by analyzing prior crystallization trials, the classification of crystallization trial images by effective feature extraction, the analysis of crystal growth in time series images, the segmentation of crystal regions in images, the application of focal stacking methods for crystallization images, and the visualization of trials. Topics and features: describes the fundamentals of protein crystallization, and the sco...
This book constitutes the refereed proceedings of the 11th International Symposium on Bioinformatics Research and Applications, ISBRA 2015, held in Norfolk, VA, USA, in June 2015. The 34 revised full papers and 14 two-page papers included in this volume were carefully reviewed and selected from 98 submissions. The papers cover a wide range of topics in bioinformatics and computational biology and their applications.
This volume explores the latest integrated bioprocesses and technologies used to study the production of the target recombinant protein of therapeutic or diagnostic interest; its isolation, purification, and stabilization; and the bio-interaction and structural analyses. The chapters in this book are organized into four parts. Part One covers production methods of soluble and membrane proteins in prokaryotic and eukaryotic expression systems, such as Lactococcus lactis and Escherichia coli. Part Two describes traditional and novel approaches for recombinant protein purification and stabilization, and buffers and additives. Part Three discusses automated methods in structural biology based on...
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In a microgravity experiment, the conditions prevalent in fluid phases can be substantially different from those on the ground and can be exploited to improve different processes. Fluid physics research in microgravity is important for the advancement of all microgravity scients: life, material, and engineering. Space flight provides a unique laboratory that allows scientists to improve their understanding of the behaviour of fluids in low gravity, allowing the investigation of phenomena and processes normally masked by the effects of gravity and thus difficult to study on Earth. Physics of Fluids in Microgravity provides a clear view of recent research and progress in the different fields of fluid research in space. The topics presented include bubles and drops dynamics, Maragoni flows, diffustion and thermodiffusion, solidfication,a nd crystal growth. The results obtained so far are, in some cases, to be confirmed by extensive research activities on the International Space station, where basic and applied microgravity experimentation will take place in the years to come.