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Yeast Metabolic Engineering
This second edition volume expands on the previous edition with new and updated chapters on the latest developments in the study of yeast within the biotechnology field. The chapters in this book cover topics such as transformation protocols for genetic engineering of Saccaromyces cerevisiae and Komagataella spp.; an overview of selection markers, promoters, and strains used for metabolic engineering of S. cerevisiae, P. pastoris, and Z. bailii; the use of yeast in CRISPR/Cas9 technology; tools to study metabolic pathway in Yarrowia lypolitica; and a discussion on the “universal expression system” that is applied in a broad spectrum of fungal species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Yeast Metabolic Engineering: Methods and Protocols, Second Edition is a valuable resource for researchers and scientists interested in learning more about this important and developing field.
Software Testing Strategies
Unlock the true potential of software testing to achieve seamless software performance with this comprehensive guide Key Features Gain a solid understanding of software testing and master its multifaceted strategies Empower yourself to effectively overcome software testing challenges Develop actionable real-world testing skills for succeeding in any role Purchase of the print or Kindle book includes a free PDF eBook Book DescriptionIn today’s world, software is everywhere—from entertainment apps to mission-critical systems that support our health, finance, and infrastructure. Testing plays a vital role in ensuring these systems work reliably. Whether you're a software developer, hobbyist...
Persecution
In this New York Times bestseller, David Limbaugh exposes the liberal hypocrisy of promoting political correctness while discriminating against Christianity. From the elimination of school prayer to the eradication of the story of Christianity from history textbooks, this persuasive book shows that our social engineers inculcate hostility toward this religion and its values in the name of "diversity," "tolerance," and "multiculturalism." Through court cases, case studies, and true stories, Limbaugh details the widespread assault on the religious liberties of Christians in America today and urges believers to fight back in order to restore their First Amendment right of religious freedom.
Wisconsin Votes
This is the first full history of voting in Wisconsin from statehood in 1848 to the present. Fowler both tells the story of voting in key elections across the years and investigates electoral trends and patterns over the course of Wisconsin's history. He explores the ways that ethnic and religious groups in the state have voted historically and how they vote today, and he looks at the successes and failures of the two major parties over the years. Highlighting important historical movements, Fowler discusses the great struggle for women's suffrage and the rich tales of many Wisconsin third parties--the Socialists, Progressives, the Prohibition Party, and others. Here, too, are the famous politicians in Wisconsin history, such as the La Follettes, William Proxmire, and Tommy Thompson. Winner, Award of Merit for Leadership in History, American Association for State and Local History
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