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This volume provides an overview of RNA bioinformatics methodologies, including basic strategies to predict secondary and tertiary structures, and novel algorithms based on massive RNA sequencing. Interest in RNA bioinformatics has rapidly increased thanks to the recent high-throughput sequencing technologies allowing scientists to investigate complete transcriptomes at single nucleotide resolution. Adopting advanced computational technics, scientists are now able to conduct more in-depth studies and present them to you in this book. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and equipment, step-by-step, readily reproducible bioinformatics protocols, and key tips to avoid known pitfalls. Authoritative and practical, RNA Bioinformatics seeks to aid scientists in the further study of bioinformatics and computational biology of RNA.
A wild seascape, a distant island, a full moon. Gradually the island grows nearer until we land on a primeval wilderness, rich in vegetation and huge, strange beasts. Time passes and things do not go well for the island. Civilization rises as towers of stone and metal and smoke, choking the undergrowth and the creatures who once moved through it. This is not a happy story and it will not have a happy ending. Working in his distinctive, monochromatic linocut style, Stanley Donwood carves out a mesmerising, stark parable on environmentalism and the history of humankind.
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This text brings together a wide range of facts, figures and directory information. It is the essential what's-what and who's-who of the world today, providing everything a reader might wish to know in a world driven by information.