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Genome Editing Tools and Gene Drives
Genome-editing methods are becoming routine tools for molecular and cell biologists. Such tools include ZFNs, CRISPR, megaTALs and TALENs. These tools are revolutionizing the creation of precisely manipulated genomes to modify the characteristics of organisms or cells. Additionally, gene drives have altered the way we understand inheritance laws. They give us the ability to have total control of the inheritance of traits of choice and importance. This succinct volume summarizes the history, principles and applications – as well as the advantages and disadvantages – of each of these tools and various kinds of gene drives. The book is part of a program to produce books helpful to students ...
Vermicomposting for Sustainable Food Systems in Africa
This edited book collates the research done mainly in Africa on vermicomposting and related technologies that can assist African smallholder farmers in making nutrient rich organic fertilizers from their animal and crop residue wastes. Information on the development of organic sources of liquid fertilizer development for hydroponics is also presented. The book presents research findings on vermicomposting in a simplified way that will allow farmers and extension workers to adopt the indicated technologies. The book also covers critical aspects of standardization of vermicompost preparation, earthworm biology, nutrient enrichment of composts and organic liquid fertilizer production. Vermicomp...
The Guinea Pig Model
This book summarizes the development and statistical validation of a guinea pig model as an alternative for potency testing of the viral antigens included in combined vaccines applied in cattle to control the respiratory, reproductive, and neonatal calf diarrhea syndromes. The model allows, in one serum sample, to test the vaccine quality for all the viral antigens included in aqueous as well as in oil-adjuvanted formulations of bovine vaccines. The methodology proposed for the control of bovine herpes virus, parainfluenza, and rotavirus were recommended by CAMEVET as guidelines for the 30 countries in the forum, including the US. Key Features Reviews combined vaccines used for cattle Summarizes animal models used for vaccine testing Focuses on bovine herpesviruses, rotaviruses, parainfluenza, and bovine viral diarrhea virus Provides guidance on the effectiveness of the Guinea Pig model for testing vaccine immunogenicity
Chemical agriculture and pollinators: signs of a Planet in danger
Bees, that have inhabited the Planet for over 100 million years, are the common thread that tells the story of various ecological challenges such as the reduction of biodiversity, climate change, soil degradation, and energy transition. Paradoxically, agriculture is one of the major causes of irreversible and, therefore, unsustainable changes such as global warming and the extinction of pollinators from which it derives its benefits and wealth. The massive use of fossil fuels, the distribution of poisons such as pesticides (persistent, toxic, and bioaccumulative), the loss of fertility in monocultures of plants selected to satisfy economic needs, are some of the main causes of an ecologically unsustainable food production system. The book tries to show a different vision of the World we are building, a story of backstories and underestimated dangers. This book received two prizes: "Steli di Pace" (Stems of Peace) by the Union of European Journalists and Communicators in 2023 and it was the winner of the "Concorso Nazionale per la divulgazione scientifica Kerit-LC Edizioni" (National Competition for science dissemination) in 2024.
Vaccine Efficacy Evaluation
Testing the immunogenicity, protective efficacy and safety in animal models is a crucial step in vaccine development. Pigs raised in germ-free environments, called gnotobiotic (Gn) pigs, are one of the most useful animal models for testing vaccines. The Gn pig model is a widely accepted model for studying pathogenesis and immunity and an ideal model for pre-clinical testing for the safety and efficacy of enteric viral vaccines. Through these studies and others, the Gn pig model has been established as the most reliable animal model for pre-clinical evaluation of human rotavirus and norovirus vaccines. This book provides detailed information on establishing Gn pig models, determining a proper...
Virus Detection
Viruses do not behave as other microbes; their life cycles require infecting healthy cells, commandeering their cellular apparatus, replicating and then killing the host cell. Methods for virus detection and identification have been developed only in the past few decades. These recently developed methods include molecular, physical, and proteomic techniques. All these approaches (Electron Microscopy, Molecular, Direct Counting, and Mass Spectrometry Proteomics) to detection and identification are reviewed in this succinct volume. It is written in approachable language with enough detail for trained professionals to follow and want to recommend to others. Key Features Covers common detection methods Reviews the history of detection from antiquity to the present Documents the strengths and weaknesses of various detection methods Describes how to detect newly discovered viruses Recommends specific applications for clinical, hospital, environmental, and public health uses
Ronald Reagan
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Genome Editing Tools and Gene Drives
This book succinctly reviews and describes genome editing tools such as ZFNs, CRISPR, megaTALs and TALENs. It covers the history, principles, applications and the advantages and disadvantages of each tool. The book provides an approachable outline enhancing science education in schools and universities by demystifying genome editing technologies.
Introduction to Pharmaceutical Biotechnology, Volume 1
Animal biotechnology is a broad field including polarities of fundamental and applied research, as well as DNA science, covering key topics of DNA studies and its recent applications. In Introduction to Pharmaceutical Biotechnology, DNA isolation procedures followed by molecular markers and screening methods of the genomic library are explained in detail. Interesting areas such as isolation, sequencing and synthesis of genes, with broader coverage of the latter, are also described. The book begins with an introduction to biotechnology and its main branches, explaining both the basic science and the applications of biotechnology-derived pharmaceuticals, with special emphasis on their clinical...
