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Photosynthesis Research Protocols
Photosynthesis is one of the most important biological phenomena on earth. The conversion of sunlight by photosynthetic organisms supplies most of the energy required to develop and sustain life on the planet. Photosynthesis is not only at the heart of plant bioenergetics, it is also fundamental to plant prod- tivity and biomass. Photosynthetic carbon fixation and oxygen evolution - rectly intervene in many environmental, including the global atmospheric CO 2 level and global climate. Therefore, it is not surprising that a large effort is devoted to photosynthesis research. Several biochemical methods of isolation, treatment, and analysis have been developed to fulfill the needs of photosynt...
The Opposite Way
December 1941. A Pan American Flying Boat takes off from San Francisco bound for Honolulu. As it approaches Hawaii, it suddenly must divert to an alternate site due to the Japanese attack on Pearl Harbor. It eventually reaches New Zealand, but finds itself unable to return the United States mainland due to the presence of the hostile Japanese fleet. To safely return home, it must circumnavigate the globe westward, "The Opposite Way." Thus begins the epic journey for the passengers and crew of the 'Caledonia Clipper' as she travels uncharted waters and visits unfamiliar destinations under the cover of radio silence in an effort to escape the Japanese war machine at the onset of World War II. ...
Signal Transduction Protocols
In 1995, Signal Transduction Protocols, edited by David A. Kendall and Stephen J. Hill, was published in the Methods in Molecular Biology series. This second edition represents an update to that previous work with an emp- sis on new methodologies that have developed in the last few years. The goal, then and now, is to provide procedures written by experts with first-hand ex- rience in a detail that goes far beyond what is generally encountered in the “methods” section of most journals and thus actually permits a particular p- cedure to be replicated. In addition, we have had as a secondary goal the id- tification of protocols for the assay of general classes of signal transduction compon...
Mad Dogs, Midgets and Screw Jobs
A fascinating history of one of the hottest wrestling territories of all time Montreal was the proving ground for some of the biggest names in wrestling, including Andre the Giant, Abdullah the Butcher, and the infamous Mad Dog Vachon; it was the site of the first midget battle ever; and made famous worldwide for the infamous Survivor Series screw job that saw Vince McMahon, the Heartbreak Kid Shawn Michaels, and Bret Hart create the "attitude" that reshaped the business. Mad Dogs, Midgets and Screw Jobs is the ultimate guide to Montreal's legendary place in professional wrestling history. Get the lowdown on all the major wrestlers who made their name in the territory, from Yvon Robert, the Rougeaus, and Gino Brito to edouard Carpentier. With a detailed account of the promotional war between the Rougeaus' AllStar Wrestling and the Vachons' Grand Prix, a complete history of how wrestling developed on Montreal TV, and an investigation of the murder of Dino Bravo, this book demonstrates how much of what has happened in wrestling, just may have happened first in Montreal.
Chlorophyll a Fluorescence
Chlorophyll a Fluorescence: A Signature of Photosynthesis highlights chlorophyll (Chl) a fluorescence as a convenient, non-invasive, highly sensitive, rapid and quantitative probe of oxygenic photosynthesis. Thirty-one chapters, authored by 58 international experts, provide a solid foundation of the basic theory, as well as of the application of the rich information contained in the Chl a fluorescence signal as it relates to photosynthesis and plant productivity. Although the primary photochemical reactions of photosynthesis are highly efficient, a small fraction of absorbed photons escapes as Chl fluorescence, and this fraction varies with metabolic state, providing a basis for monitoring q...
Apoptosis Methods and Protocols
The most fundamental question facing each and every cell within an org- ism is to survive or to die. Cell death is required for normal function; some estimates suggest that as many as one million cells undergo cell death every second in the adult human body. Almost all cells undergoing physiological, or programmed, cell death, independent of cell type, manifest a stereotypic p- tern of morphological changes termed apoptosis. Typically, apoptotic cells d- play shrinkage, membrane blebbing, chromatin condensation, and nuclear fragmentation. The integrity of the cell membrane is not lost during apoptosis and so avoids eliciting the inflammatory response that would have been caused by the spilla...
Cell Cycle Control
The fundamental question of how cells grow and divide has perplexed biologists since the development of the cell theory in the mid-19th century, when it was recognized by Virchow and others that “all cells come from cells.” In recent years, considerable effort has been applied to the identification of the basic molecules and mechanisms that regulate the cell cycle in a number of different organisms. Such studies have led to the elucidation of the central paradigms that underpin eukaryotic cell cycle control, for which Lee Hartwell, Tim Hunt, and Paul Nurse were jointly awarded the Nobel Prize for Medicine and Physiology in 2001 in recognition of their seminal contributions to this field. The importance of understanding the fundamental mechanisms that modulate cell division has been reiterated by relatively recent discoveries of links between cell cycle control and DNA repair, growth, cellular metabolism, development, and cell death. This new phase of integrated cell cycle research provides further challenges and opportunities to the biological and medical worlds in applying these basic concepts to understanding the etiology of cancer and other proliferative diseases.
Oligonucleotide Synthesis
A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.
