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Radioligand binding in situ is the most sensitive method for detecting available receptors; the success and reliability of this approach are determined by a number of technical requirements and constraints. Based on the authors' fifteen years of experience in the field, Visualization of Receptors In Situ: Applications of Radioligand Binding explici
Although the polymerase chain reaction has revolutionized genetic analysis by amplifying rare nucleic acid sequences, the in situ application is the only method that allows the localization of amplified signal within tissue structure. The applications of in situ polymerase chain reaction have greatly enhanced the field of investigation in many disc
Visualization of nuclear components has added tremendously to our understanding of the nucleus and its chemistry. The fluorescence approach is considered the present-day standard for comprehending nuclear organization. Imaging of Nucleic Acids and Quantitation in Photonic Microscopy provides new technologies in fluorescence microscopy and image cyt
Visualization of nucleic acids has become indispensable to studying cells, tissues, and organisms. Certain techniques even permit quantification of DNA and/or RNA distribution in tissues, but few current analytical books cover the numerous methods for DNA and RNA visualization. This book provides insight into several classic techniques, histologica
In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods developed for the ultrastructural visualization
In Situ hybridization allows the visualization of specific DNA/RNA sequences in individual cells in tissue sections, single cells, or chromosome preparations, and is an especially important method for studying DNA and RNA in heterogeneous cell populations. This book delves into in situ hybridization methods through the use of light microscopy used
Histochemistry and cytochemistry are important fields for studying the inner workings of cells and tissues of the body. While visualization techniques have been in use for many years, new methods of detection developed recently help researchers and practitioners better understand cell activity. Histochemical and Cytochemical Methods of Visualizatio