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This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing.
First multi-year cumulation covers six years: 1965-70.
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The second of a series of Yearbooks in the Work Life 2000 programme, preparing for the Work Life 2000 Conference in Malmö 22 - 25 January 2001, as a part of the Swedish Presidency of the European Union
Summary of a workshop sponsored by OECD's Road Research Program.
The analysis of DNA sequence polymorphisms and mutations is of central importance in understanding biological systems. This book is devoted to the experimental analysis of DNA and presents easy-to-follow protocols. Various techniques from the simple to the highly complex are detailed in this volume, providing a wide spectrum of available methods and practical advice. The methods are described in terms of: History and background Principles and theory Equipment and reagents Protocols Troubleshooting Applications Improvements Results Comparisons with other methods Future prospects and developments This is an essential manual for researchers working in human, animal, or plant molecular genetics and is particularly valuable for hospital and commercial laboratories.
It has been around since the first rear-impact automobile accident and it will continue to be a problem as long as humans have large, heavy heads perched on slender, highly mobile cervical spines. The subject is whiplash, and some of the brightest minds on the topic gathered in Banff, Alberta, Canada, for the Eighth International Symposium by the Physical Medicine Research Foundation. Editor Dr. Murray E. Allen, Chairman of the Symposium, has collected the findings in Musculoskeletal Pain Emanating From the Head and Neck: Current Concepts in Diagnosis, Management, and Cost Containment to help physicians, physical therapists, chiropractors, and researchers better understand “the new whiplas...
PÃ¥ jakt efter en större förstÃ¥else för spelet kring de moderna antidepressiva medicinerna tar Ingrid Carlberg läsaren med frÃ¥n Sigmund Freud i artonhundratalets Wien till PR-strategier och marknadsplaner i nutidens multinationella läkemedelsbolag. Uppskattningsvis 600 000 svenskar tar dagligen nÃ¥gon av de moderna antidepressiva medicinerna. MÃ¥nga vittnar om att de sannolikt inte varit i livet idag om läkemedlen inte hade funnits. Andra upplever att problemen blivit större med än utan medicinerna. I boken berättar nÃ¥gra av dem sin historia. FÃ¥ läkemedel har som "lyckopillren" väckt sÃ¥ starka känslor i samhällsdebatten och lyfts till sÃ¥ tydliga kulturella symboler för vÃ...
New technologies and the growing flow of information create new conditions for individuals who use these technologies in the work place. The existence and application of modern IT systems can result in new forms of work, tasks that have actually emerged as a result of modern computer and other systems. This third Work Life 2000 Yearbook is pan-European in nature, and provides the researcher with valuable source material relating to the EU's response to the changing working environment.