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This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing.
First multi-year cumulation covers six years: 1965-70.
The authoritative guide on protein purification—now completely updated and revised Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch. Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes: High-resolution reversed-phase liquid chromatography Electrophoresis in gels Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients Affinity ligands from chemical and biological combinatorial libraries Membrane separations Refolding of inclusion body proteins from E. coli Purification of PEGylated proteins High throughput screening techniques in protein purification The history of protein chromatography
In this challenging book, the authors demonstrate that economists tend to misunderstand capital. Frank Knight was an exception, as he argued that because all resources are more or less durable and have uncertain future uses they can consequently be classed as capital. Thus, capital rather than labor is the real source of creativity, innovation, and accumulation. But capital is also a phenomenon in time and in space. Offering a new and path-breaking theory, they show how durable capital with large spatial domains — infrastructural capital such as institutions, public knowledge, and networks — can help explain the long-term development of cities and nations.
It has been around since the first rear-impact automobile accident and it will continue to be a problem as long as humans have large, heavy heads perched on slender, highly mobile cervical spines. The subject is whiplash, and some of the brightest minds on the topic gathered in Banff, Alberta, Canada, for the Eighth International Symposium by the Physical Medicine Research Foundation. Editor Dr. Murray E. Allen, Chairman of the Symposium, has collected the findings in Musculoskeletal Pain Emanating From the Head and Neck: Current Concepts in Diagnosis, Management, and Cost Containment to help physicians, physical therapists, chiropractors, and researchers better understand “the new whiplas...
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The second of a series of Yearbooks in the Work Life 2000 programme, preparing for the Work Life 2000 Conference in Malmö 22 - 25 January 2001, as a part of the Swedish Presidency of the European Union
Summary of a workshop sponsored by OECD's Road Research Program.
New technologies and the growing flow of information create new conditions for individuals who use these technologies in the work place. The existence and application of modern IT systems can result in new forms of work, tasks that have actually emerged as a result of modern computer and other systems. This third Work Life 2000 Yearbook is pan-European in nature, and provides the researcher with valuable source material relating to the EU's response to the changing working environment.