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This volume details generation of gene-edited cell lines and organisms as models for human diseases, pest control, and large animal welfare and production outcomes. Chapters guide readers through gene regulation, editing, screening of cell lines, genome editing, and an overview of the tools for efficient genome editing including; ZFNs, TALENs, and CRISPR. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step- by-step, readily reproducible protocols. Authoritative and cutting-edge, Applications of Genome Modulation and Editing aims to be a useful and practical guide for researchers to commence or advance their study in this field.
Collects Uncanny X-Men (1981) #168-176. Is the Phoenix rising again? Chris Claremont and Paul Smith's classic UNCANNY X-MEN arc puts the team through major changes - and a test of faith! It's the start of a beautiful friendship between Kitty Pryde and Lockheed, but why does Kitty think Professor Xavier is a jerk? Storm battles Callisto, leader of the outcast mutant Morlocks - but why is Storm so unsettled, and what will she do to resolve her inner conflicts? Meanwhile, Rogue joins the X-Men, but how will the team react to the addition of their former foe? Wolverine and Mariko Yashida are engaged - but surprises await the X-Men in Japan! And Cyclops has a new love in Madelyne Pryor…but clues that Dark Phoenix is returning begin to point heartbreakingly close to home! Experience one of the X-Men's most beloved storylines!
Collects Uncanny X-Men (1981) #168-175, X-Men Annual (1970) #7, Marvel Graphic Novel (1982) #5, Wolverine (1982) #1-4. By 1982, the Uncanny X-Men had become the biggest franchise in comic books. Chris Claremont's writing rose to find perhaps his most poignant and challenging expression of the mutant metaphor in "God Loves, Man Kills," while his work with Frank Miller on Wolverine skyrocketed the character's popularity. With the culmination of Paul Smith's classic run - featuring the growing romance between Kitty Pryde and Colossus, the first appearance of Callisto and the Morlocks, Rogue's debut as an X-Man.
One day a woman makes a list of what she wants to do, like, dump her husband, make new friends, do new things, etc.
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This volume gathers research at the intersection of art and the interdisciplinary humanities to develop an understanding of media assemblages that insist on the generativity of their situatedness within ecologies of practice. These contributions propose media assemblages that enlarge the time and space for co-compositions between media and bodies that reshape subjective, perceptual, and affective registers of experience. Media assemblages include photography, performance, criticism, curation, installation, animation, collage, video and VR, as well as archival and somatic practices. Research as a form of practice is a key orientation in this volume since it offers a means of engaging the worl...
Learn the official language of Kenya and Tanzania Swahili For Dummies will teach you the basics of Swahili, so you can start conversing in Africa’s language of commerce. This book introduces you to the foundations of Swahili grammar and enables you to engage in basic conversations. With the simplified Dummies learning process, you’ll quickly get a grasp on the language, without complex terms and confusing explanations. You’ll also move through the book at a comfortable pace, so you’ll be familiar with what you’ve learned before moving on to more complex stuff. Focus on communication and interaction in everyday situations, so you can actually use the language you’re studying—right away. Understand the basics of Swahili Learn everyday words and phrases Gain the confidence to engage in conversations in Swahili Communicate while traveling and talk to Swahili-speaking family members Swahili For Dummies is for readers of all ages who want to learn the basics of Swahili in a no-stress, beginner-friendly way. Swahili teachers will also love sharing this practical approach with their students.
Beginning in 2018, Ahmed M. Badr—an Iraqi-American poet and former refugee—traveled to Greece, Trinidad & Tobago, and Syracuse, New York, holding storytelling workshops with hundreds of displaced youth: those living in and outside of camps, as well as those adjusting to life after resettlement. Combining Badr’s own poetry with the personal narratives and creative contributions of dozens of young refugees, While the Earth Sleeps We Travel seeks to center and amplify the often unheard perspectives of those navigating through and beyond the complexities of displacement. The result is a diverse and moving collection—a meditation on the concept of "home" and a testament to the power of storytelling.