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Enhancers and Promoters
This volume contains cutting-edge techniques to study the function of enhancers and promoters in depth. Chapters are divided into six sections and describe enhancer-promoter transcripts, nucleosome occupancy, DNA accessibility, chromatin interactions, protein-DNA interactions, functional analyses, and DNA methylation assays. Written in the Methods in Molecular Biology series format, chapters include comprehensive introductions, lists of the necessary materials and reagents, step-by-step laboratory protocols, and useful suggestions for troubleshooting. Authoritative and cutting-edge, Enhancers and Promoters: Methods and Protocols is a useful guide for future experiments.
In the Forge of Stalin
Gammalsvenskby is the only Swedish settlement to the east from Finland, founded in 1782. In the past of Gammalsvenskby the history of the Soviet Union, Sweden, the international communist movement and Nazi Germany combined in a bizarre form. And even when the ploughmen of the Kherson steppes did not left their native village, the great powers themselves visited them with the intention to rule forever. The history of colony is viewed through the prism of the theory of “forcednormalization” and the concept of “changes of collective identity“. The author intends to study the techniques of forced normalization and the strategy of the collective resistance. Andrej Kotljarchuk is an associ...
Biology of Trypanosoma cruzi
Trypanosoma cruzi, an important zoonotic protozoan that causes Chagas disease, affects at least 8 million people in Latin America. Chagas disease is an important life-long infection in humans that can be divided into distinct clinical stages: the acute phase, where patient symptoms can vary from asymptomatic to severe; the indeterminate form, which is usually asymptomatic; and the chronic phase, where cardiomyopathy and/or digestive megasyndromes appear. In addition to its medical importance, T. cruzi is an interesting biological model for studying processes such as: (1) cell differentiation, where a non-infective stage transforms into an infective one; (2) cell invasion, where the infective...
