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Basics of CRISPR/Cas Mediated Plant Genome Editing
CRISPR/Cas has emerged, as a powerful genetic engineering tool. It is simple yet highly affordable and robust. This book provides a basic introduction to the mechanism and architecture of the CRISPR/Cas. It enlightens the readers about the new advancements such as gene insertion, transcriptional activation, suppression, gene tagging, multiplexing, base editing, prime editing, and tissue culture free editing which makes it easier for crop improvement. With such technologies, application of genome editing has opened new paths for crop improvements and sustainable agriculture. The current book will be useful for beginners to understand these tools and how to use them in a better and more efficient way.
Molecular Approaches in Plant Biology and Environmental Challenges
This book discusses molecular approaches in plant as response to environmental factors, such as variations in temperature, water availability, salinity, and metal stress. The book also covers the impact of increasing global population, urbanization, and industrialization on these molecular behaviors. It covers the natural tolerance mechanism which plants adopt to cope with adverse environments, as well as the novel molecular strategies for engineering the plants in human interest. This book will be of interest to researchers working on the impact of the changing environment on plant ecology, issues of crop yield, and nutrient quantity and quality in agricultural crops. The book will be of interest to researchers as well as policy makers in the environmental and agricultural domains.
Plant Transformation
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Recent Trends and Techniques in Plant Metabolic Engineering
Remarkable research has yielded whole genome data in plants, resulting in the documentation of an ever-increasing number of genes, without establishing their functions. The huge data resources available at the genome, transcriptome, proteome and metabolome levels are of enormous value in the field of functional genomics. This book provides insights into interpreting the sea of data in order to understand basic and practical aspects of plant metabolic engineering. It discusses in detail ways to tap into this enormous pool of data to increase productivity, and offers information that is both interesting and necessary for exploring the manipulation of metabolic pathways. The interdisciplinary approaches presented here also serve as a source of ideas for practical applications.
Molecular Aspects of Plant Beneficial Microbes in Agriculture
Molecular Aspects of Plant Beneficial Microbes in Agriculture explores their diverse interactions, including the pathogenic and symbiotic relationship which leads to either a decrease or increase in crop productivity. Focusing on these environmentally-friendly approaches, the book explores their potential in changing climatic conditions. It presents the exploration and regulation of beneficial microbes in offering sustainable and alternative solutions to the use of chemicals in agriculture. The beneficial microbes presented here are capable of contributing to nutrient balance, growth regulators, suppressing pathogens, orchestrating immune response and improving crop performance. The book also offers insights into the advancements in DNA technology and bioinformatic approaches which have provided in-depth knowledge about the molecular arsenal involved in mineral uptake, nitrogen fixation, growth promotion and biocontrol attributes.
Metabolic Engineering for Bioactive Compounds
This book comprehensively discusses the latest research in the area of metabolic engineering. Metabolic engineering solutions for bioactive compounds are now being derived by means of heterologous gene expression, in a wide range of organisms. The book provides an overview of the model systems being employed for metabolic manipulation to yield bioactive molecules, such as single-cell proteins, antibody generation, metabolites, proteases, chaperones, therapeutic proteins, nanomaterials, polymeric conjugates, dendrimers and nanoassemblies, Escherichia coli, Agrobacterium, Saccharomyces cerevisiae and cell lines, etc. In addition, it shares insights into the scope of these methods in the areas of prevention, diagnosis and treatment of diseases, e.g. immunotherapy for curing various diseases like cancer, allergies, autoimmune diseases, etc.
