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ARE YOU READY FOR A BEAUTIFUL BREAKTHROUGH?Abuse causes damage, destruction, and devastation to every aspect of the mind, body, and soul. But through Christ you can heal and rebuild a beautiful life!Having come to terms with her own repressed childhood trauma, Clare Davy is on a mission to silence the shame in survivors and equip you with the courage to find your voice, deconstruct your messy mindsets, and live in full freedom.In The Reconstructed Woman: Blueprints for a Beautiful Life, Clare provides you with the tools to Release, Restore, and Rebuild your life through Christ. Written for those who desire healing from sexual abuse, but have no idea where to start, Clare shares personal trials and triumphs, journal prompts, bible verses, prayers, and tools for your very own transformation!No more bowing to the aftermath of abuse. Let Clare help you find your voice and break through into the beautiful after brokenness!Clare Davy is the founder of The Reconstructed Woman, a Speaker, Christian Life & Marriage Coach. To learn more about Clare please visit her website at www.claredavy.com
Leading basic researchers and clinical scientists describe in detail a wide variety of established and cutting-edge techniques they have developed to study the lifecycle and biological properties of the human papillomavirus. The authors use these readily reproducible methods, ranging from PCR to propagation of HPV in vitro, to detect and type papillomavirus infections, study the papillomavirus lifecycle, and to produce and functionally analyze papillomavirus proteins. The protocols follow the successful Methods in Molecular MedicineTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
This book examines specific techniques which can be used to explore new drug targets and the effectiveness of new antibiotics. By testing new antimicrobial agents and modified existing drugs, the most vulnerable cell processes, such as cell wall and membrane synthesis, DNA replication, RNA transcription and protein synthesis, can be better exploited. This in-depth volume, however, delves even deeper by identifying additional novel cellular targets for these new therapies. The book will provide laboratory investigators with the vital tools they need to test the antimicrobial potential of products and to curb the rise of so many infectious diseases.
A cutting-edge collection of readily reproducible molecular techniques to better understand, classify, and treat lymphoma. Among the highlights are methods to use immunoglobulin gene rearrangements as markers of clonality, to exploit patterns of somatic mutation in the variable regions to indicate at which stage transformation occurred, and to apply gene arrays to the question of biological heterogeneity in morphologically similar diseases. Research methodologies that are highly likely to become routine practice in the future, such as DNA microarray and immunoglobulin V-gene rearrangements, and measurement of minimal disease, are included. There are also molecular techniques for providing for producing novel therapeutics, such as a DNA vaccine with patient-specific sequences derived from the lymphoma in question.
Leading experts in the use of MRI explain its basic principles and demonstrate its power to understand biological processes with numerous cutting-edge applications. To illustrate its capability to reveal exquisite anatomical detail, the authors discuss MRI applications to developmental biology, mouse phenotyping, and fiber architecture. MRI can also provide information about organ and tissue function based on endogenous cantrast mechanisms. Examples of brain, kidney, and cardiac function are included, as well as applications to neuro and tumor pathophysiology. In addition, the volume demonstrates the use of exogenous contrast material in functional assessment of the lung, noninvasive evaluation of tissue pH, the imaging of metabolic activity or gene expression that occur on a molecular level, and cellular labeling using superparamagnetic iron oxide contrast agents.
A record of crests of Suffolk and Norfolk families arranged by charge or object, covering 600 years and c.8,000 names. This volume offers a comprehensive guide to the heraldry of Suffolk over more than six centuries, covering around 8,000 names and acting as a companion to the earlier Dictionary of Suffolk Arms(1965). It is the first attempt to produce an Ordinary of crests, a classification by charge or object using standardised groupings, arranged in such a manner that they may be readily identified when the name of the bearer is unknown; the usual arrangement isalphabetical by name, an Armory. Although it relates specifically to Suffolk, many crests relating to Norfolk families are given, the two counties having always been closely connected heraldically and genealogically. The book willbe of interest for all those interested in heraldry and, on a wider level, act as a handbook for the identification of crests when borne alone, on artefacts ranging from signet rings and silverware to pub signs and school uniformcrests. JOAN CORDER, the author of a Dictionary of Suffolk Arms, is an independent scholar and recognised authority on East Anglian heraldry.
A panel of internationally recognized research scientists and clinical investigators brings together a diverse collection of readily reproducible methods for identifying and quantifying a large number of specific genetic abnormalities associated with the broad spectrum of myeloid malignancies. Highlights include techniques for the detection of BCR-ABL mutations and resistance to imatinib mesylate, detection of the FIP1L1-PDGFRA fusion in idiopathic hypereosinophilic syndrome and chronic eosinophilic leukemia, classification of AML by DNA-oligonucleotide microarrays, and detection of the V617F JAK2 mutation in myeloproliferative disorders. In addition to gene rearrangments, other prognostically relevant molecular lesions such as FLT3 mutations and WT-1 overexpression are covered.
A cutting-edge collection of readily reproducible in vitro and in vivo methods to elucidate the mechanisms associated with cannabinoid function in health and disease. The techniques can be used in studies across the board from genes to behavior. The molecular neurobiological methods are invaluable in analyzing the structure, the polymorphisms, and the molecular expression of the cannabinoid receptors (CBrs), as well as their association with polysubstance abuse. There are also methods for localizing cannabinoid receptors in different systems, visualizing cannabinoid effects using brain slice imaging and elctrophysiological approaches, and designing and synthesizing cannabinoids and endocannabinoids. The protocols follow the successful Methods in Molecular MedicineTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cardiovascular disease is the leading cause of death in developed countries, but is quickly becoming an epidemic in such well-populated countries as China, India, and other developing nations. Cardiovascular research is the key to the prevention, diagnosis, and management of cardiovascular disease. Vigorous and cross-disciplinary approaches are required for successful card- vascular research. As the boundaries between different scientific disciplines, particularly in the life sciences, are weakening and disappearing, a successful investigator needs to be competent in many different areas, including genetics, cell biology, biochemistry, physiology, and structural biology. The newly developed ...