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This volume incorporates practical methods at the level of molecular, cellular, and whole organism biology in vertebrate and invertebrate models. It presents straightforward protocols written step-by-step for state-of-the-art techniques with the emphasis on single-cell resolution procedures. - Provides straightforward, current protocols and critical appraisals - Includes diverse analysis of cellular and molecular techniques - Presents everything from whole-organ cultures to electrophysiological approaches - Details a variety of methods for interfering with gene function in various species - Offers multiple illusions of in situ hybridization, immunostaining, and apoptosis
Brings together easy-to-follow protocols and practical instructions for all of the main techniques in classical embryo manipulation, from traditional embryology to cellular and molecular methods. The book includes reprints of all the stage tables in common use for the main laboratory species.
This book is the product of a NATO Advanced Study Institute of the same name, held at the Anargyrios and Korgialenios School on the island of Spetsai, Greece, in September 1994. The institute considered the molecular mechanisms which generate the body plan during vertebrate embryogenesis. The main topics discussed included: commitment and imprinting during germ cell differentiation; hierarchies of inductive cell interactions; the molecular functioning of Spemann's organizer and formation of embryonic axes; the extracellular matrix and the cytoskeleton in relation to morphogenesis and cell migration; neurogenesis and patterning of the neuraxis; the regulation of pattern formation by Hox genes...
Organizers in Development, Volume 157 in the Current Topics in Developmental Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as The organizer: what it meant, and means, to developmental biology, Organizers/signaling regions that pattern the limb, Organizers/signaling regions in the branchial arches, The notochord/floor plate as a possible organizer, Transport and gradient formation of Wnt and Fgf in the early zebrafish gastrula, and more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Current Topics in Development Biology series - Updated release includes the latest information on Organizers in Development
During gastrulation, tissue layers are formed and the overall body plan is established. This book is the definitive guide to this vitally important period in embryonic development, providing authoritative and up to date information that includes the first comprehensive interspecies comparison, cell movements and patterning events, the roles of individual genes and gene families, and the evolution of gastrulation.
Collects articles discussing the specialization of cells, the processes of differentiation that occur in plant and animal cells, and how artificial differentiation can be used and the ethical questions it raises.
This revised edition will continue to serve as the most complete and up-to-date guide to the use of the avian embryo in studies of vertebrate development. It will include new approaches to analysis of the chick genome, gene knock-out studies using RNA interference, morpholinos, and other cutting edge techniques. As with the original edition, emphasis has been placed on providing practical guidance, highlighting potentials and pitfalls of all key cell biological and embryological techniques. *fully revised second edition *organized into basic and advanced Methods *new section on Functional Genomics
Cilia--the tiny hairlike structures on the surface of cells—-have recently been identified as playing a role in a variety of disease and developmental disorders. Absent or defective cilia in certain cells can cause infertility, blindness, kidney disease, and lung disease. This volume presents recent findings in cilia research and current thought on the role of cilia in disease and developmental abnormalities.
The developing heart is the first functional organ, and its proper formation and function is essential for life. This volume compiles the exciting new advances that have been made in understanding the regulation of heart development, the genes and pathways involved, and the impact these have in heart disease. A stellar collection of scientists tackles an important facet of heart development, providing a comprehensive set of reviews. Cutting-edge science Authority of contributors Exciting field of research
This Series provides a comprehensive survey of the major topics in the field of developmental biology. The volumes are valuable to researchers in animal and plant development, as well as to students and professionals who want an introduction to cellular and molecular mechanisms of development. The Series has recently passed its 30-year mark, making it the longest-running forum for contemporary issues in developmental biology.