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The Emperor's Men 8: Stormy Heavens
Stormy times are coming. The messengers of the gods establish their rule over Mutal, the metropolis of the Maya, and continue their campaign against the neighboring cities. Fear and a spirit of resistance have awakened and plant a new idea of cooperation in the heads of the Mayan kings who do not want to surrender without a fight. But even within the group of stranded people, the captain's course is being questioned more and more. When a Roman expedition finally lands from distant Europe and an ambassador from Teotihuacán is interested in the developments, the centuries-old balance of power in Central America threatens to collapse.
Pharmacogenomics
Expert researchers and physician/clinicians describe in detail the newest and most commonly used technologies today in this rapidly advancing field. The authors provide readily reproducible methods for assessing the functional consequences of a certain polymorphism, evaluate the variety of genotyping platforms currently available, and discuss the management of pharmacogenomic information. Highlights include techniques for making a snapshot of the allele-specific variation in human gene expression, genome wide analysis of allele-specific expression using oligo microarrays, in vivo assays with HaploChIP, SNP genotyping in DNA pools, and PharmGKB, the pharmacogenetics and pharmacogenomics knowledge base. The methodologies for genotyping include denaturing high-performance liquid chromarography, pyrosequencing, kinetic-fluorescence detection, mass spectrometry, and TaqMan assay for insertion/deletions.
Algorithms in Bioinformatics
This book constitutes the refereed proceedings of the Third International Workshop on Algorithms in Bioinformatics, WABI 2003, held in Budapest, Hungary, in September 2003. The 36 revised full papers presented were carefully reviewed and selected from 78 submissions. The papers are organized in topical sections on comparative genomics, database searching, gene finding and expression, genome mapping, pattern and motif discovery, phylogenetic analysis, polymorphism, protein structure, sequence alignment, and string algorithms.
The Emperor's Men 9: High Tides
The empire of Mutal is growing, and with it the number of its opponents. While a large alliance of free Mayan cities forms to put a stop to the expansion of the messengers of the gods in harmony with the mighty Teotihuacán, the Roman expedition tries to contact the mainland Mayans and find out more about the time travellers from Japan. But events quickly escalate and many who thought they were in control are taught otherwise. Conflicts escalate, events get out of control and Romans, Japanese and Mayans are caught in heavy tides ...
The Emperor's Men 3: Passage
The victory over the Goths has only brought temporary peace to the Roman Empire. Unrest and intrigues threaten to jeopardize the achievements made. The time travelers from the Saarbrücken not only help to reform Empire but also have to take first steps against the threat posed by the Huns. An expedition is sent to Africa to spread new ideas in the Empire and begin the search for profitable commodities. But the opponents of the new order are not asleep and begin their preparations to restore the old ways - and this with the help of a man who was believed to be dead.
DNA Arrays
Microarray technology provides a highly sensitive and precise te- nique for obtaining information from biological samples, with the added advantage that it can handle a large number of samples simultaneously that may be analyzed rapidly. Researchers are applying microarray technology to understand gene expression, mutation analysis, and the sequencing of genes. Although this technology has been experimental, and thus has been through feasibility studies, it has just recently entered into widespread use for advanced research. The purpose of DNA Arrays: Methods and Protocols is to provide instruction in designing and constructing DNA arrays, as well as hybridizing them with biological samples ...
Molecular Analyses
DNA and RNA extraction methods from a variety of tissues and samples are now routine, including extraction from single cells. Many methods are now automated. Sequencing efficiency has reached the point where it is now possible to obtain gigabases of data, both quickly and inexpensively. Such methods permit the identification of gene versions, including those associated with disease (e.g. small nucleotide polymorphism analyses, or SNPs). The general public as well as clinicians can now access a wide variety of literature on the molecular bases of diseases, allowing them to better assess disease risks and treatments. This volume concentrates on medically-focused methods, and therefore the majo...
Plant Genotyping II
Describes some of the developments in the field of Plant Genotyping, focusing on single nucleotide polymorphism (SNPs). This book covers the discovery, analysis and uses of SNPs, and examines other approaches to plant genotyping.
Single Nucleotide Polymorphisms
A comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.
