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Antifungal Agents
A collection of state-of-the-art molecular methods for studying antifungal resistance, for discovering and evaluating both new and existing antifungal drugs, and for understanding the host response and immunotherapy of such agents. The protocols follow the successful Methods in Molecular MedicineTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Antifungal Agents: Methods and Protocols offers clinician-scientists, microbiologists and molecular biologists the productive tools they need today to understand and successfully develop new therapeutic agents for yeast, mold, and fungal infections.
Pharmacokinetics in Drug Discovery and Development
Pharmacokinetics has evolved from its origin into a complex discipline with numerous subspecialties and applications in patient management, drug development, and regulatory issues. This expansion has made it difficult for any one individual to become a full-fledged expert in all areas. Fulfilling the need for a wide-ranging guide to the many existi
Textbook of Therapeutics
The contributors to this volume deliver information on latest drug treatments and therapeutic approaches for a wide range of diseases and conditions. Coverage includes discussion of racial, ethnic, and gender differences in response to drugs and to biotechnical, pediatric and neonatal therapies.
Pharmacotherapy
None
Marijuana and Cannabinoid Research
A cutting-edge collection of readily reproducible in vitro and in vivo methods to elucidate the mechanisms associated with cannabinoid function in health and disease. The techniques can be used in studies across the board from genes to behavior. The molecular neurobiological methods are invaluable in analyzing the structure, the polymorphisms, and the molecular expression of the cannabinoid receptors (CBrs), as well as their association with polysubstance abuse. There are also methods for localizing cannabinoid receptors in different systems, visualizing cannabinoid effects using brain slice imaging and elctrophysiological approaches, and designing and synthesizing cannabinoids and endocannabinoids. The protocols follow the successful Methods in Molecular MedicineTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Tissue Engineering
Features: Leading experts present their own most recent advances, Includes a wide spectrum of methods representing tissue engineering in many diverse disciplines, Supplies an understanding of diverse technologies and methods.
Bone Marrow and Stem Cell Transplantation
This volume is a compendium of cutting-edge molecular methods for the successful transplantation of hematopoietic stem cells. The contributors are world-renown leaders in the field. They describe promising tools for stem cell transplant research models, such as in vivo bioluminescence imaging. They discuss HLA typing, PCR-SSP typing, and HLA antigens. This volume is an invaluable source for biochemists, molecular biologists, and clinicians.
Magnetic Resonance Imaging
Leading experts in the use of MRI explain its basic principles and demonstrate its power to understand biological processes with numerous cutting-edge applications. To illustrate its capability to reveal exquisite anatomical detail, the authors discuss MRI applications to developmental biology, mouse phenotyping, and fiber architecture. MRI can also provide information about organ and tissue function based on endogenous cantrast mechanisms. Examples of brain, kidney, and cardiac function are included, as well as applications to neuro and tumor pathophysiology. In addition, the volume demonstrates the use of exogenous contrast material in functional assessment of the lung, noninvasive evaluation of tissue pH, the imaging of metabolic activity or gene expression that occur on a molecular level, and cellular labeling using superparamagnetic iron oxide contrast agents.
Placenta and Trophoblast
The aim of the two-volume set of Placenta and Trophoblast: Methods and Protocols is to offer contemporary approaches for studying the biology of the placenta. The chapters contained herein also address critical features of the female organ within which the embryo is housed, the uterus, and some aspects of the embryo–fetus itself, particularly those of common experimental animal models. In keeping with the organization used effectively in other volumes in this series, each chapter has a brief introduction followed by a list of required items, protocols, and notes designed to help the reader perform the expe- ments without difficulty. In both volumes, sources of supplies are given and illust...
Chemosensitivity
A state-of-the art collection of readily reproducible laboratory methods for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors. Chemosensitivity, Volume 1: In Vitro Assays provides a panel of 16 in vitro measures of chemosensitivity in adherent and non-adherent cells for single agents and combinations of agents. In addition to immunohistochemical and imaging approaches, these assays include clonogenic, colorimetric, fluorometric, and physiological assays. Highlights include image analysis to assess drug sensitivity, high throughput approaches using green fluorescent protein, DIMSCAN (a microcomputer fluorescence-based assay), and the ChemoFx assay used in biotechnology. A companion volume, Volume 2: In Vivo Models, Imaging, and Molecular Regulators, provides protocols for classifying tumors into response categories and customizing chemotherapy regimens to individual patients.
