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Single Molecule Analysis
This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters in this book are organized into four parts, and cover topics such as optical tweezers; single-molecule fluorescence tools; atomic force microscopy; magnetic tweezers; applications to virus protein shells, unfolding of proteins, nucleic acids, motor proteins, in vivo and in vitro; and protocols to establish specific surface interactions and perform force calibration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Single Molecule Analysis: Methods and Protocols, Third Edition is a valuable resource for all researchers who want to learn more about this exciting and still expanding field. Chapters 2, 7, 8, 9, 12, 18, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Single Molecule Tools, Part B: Super-Resolution, Particle Tracking, Multiparameter, and Force Based Methods
Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part B: Super-Resolution, Particle Tracking, Multiparameter, and Force Based Methods captures a snapshot of this vibrant, rapidly expa...
Optical Tweezers
This detailed volume explores a wide variety of techniques involving optical tweezers, a technology that has become increasingly more accessible to a broad range of researchers. Beginning with recent technical advances, the book continues by covering the application of optical tweezers to study DNA-protein interactions and DNA motors, protocols to perform protein (un)folding experiments, the application of optical tweezers to study actin- and microtubule-associated motor proteins, and well as protocols for investigating the function and mechanical properties of microtubules and intermediate filaments, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Optical Tweezers: Methods and Protocols, Second Edition serves as an ideal resource for expanding the accessibility and use of optical traps by scientists of diverse disciplines.
Single Molecule Biology
Single molecule techniques, including single molecule fluorescence, optical tweezers, and scanning probe microscopy, allow for the manipulation and measurement of single biological molecules within a live cell or in culture. These approaches, amongst the most exciting tools available in biology today, offer powerful new ways to elucidate biological function, both in terms of revealing mechanisms of action on a molecular level as well as tracking the behaviour of molecules in living cells. This book provides the first complete and authoritative treatment of this rapidly emerging field, explicitly from a biological perspective.The contents are organized by biological system or molecule. Each c...
Microtubules, in vitro
There continues to be intense interest in the microtubule cytoskeleton; the assembly, structure and regulation of microtubules; and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of these issues driven immensely by developments in optical imaging and tracking techniques. This volume (complemented by the forthcoming companion volume by Cassimeris and Tran) brings together current research and protocols in the field of microtoubules in vitro and will serve as a valuable tool for cell biologists, biophysicists and pharmacologists who study the microtubule cytoskeleton, as well as for researchers in the biomedical and biotechnology communities with interest in developing drugs that target microtubules, MAPS and motors. - Chapters reflect both experimental procedures and new developments in the field of microtubule in vitro research - Combines classical approaches and modern technologies - Presents easy-to-use protocols and thorough background information, compiled by leaders in the field
Photosynthesis :
Photosynthesis is a process on which virtually all life on Earth depends. To answer the basic questions at all levels of complexity, from molecules to ecosystems, and to establish correlations and interactions between these levels, photosynthesis research - perhaps more than any other discipline in biology - requires a multidisciplinary approach. Congresses probably provide the only forums where progress throughout the whole field can be overviewed. The Congress proceedings give faithful pictures of recent advances in photosynthesis research and outline trends and perspectives in all areas, ranging from molecular events to aspects of photosynthesis on the global scale. The Proceedings Book, a set of 4 (or 5) volumes, is traditionally highly recognized and intensely quoted in the literature, and is found on the shelves of most senior scientists in the field and in all major libraries.
Biological Motion
A captivating exploration of the changing definitions of life in biology Biological Motion studies the foundational relationship between motion and life. To answer the question, “What is Life?,” prize-winning historian of science Janina Wellmann engages in a transdisciplinary investigation of motion as the most profound definition of living existence. For decades, information and structure have dominated the historiography of the life sciences with its prevailing focus on DNA structure and function. Now more than ever, motion is a crucial theme of basic biological research. Tracing motion from Aristotle’s animal soul to molecular motors, and from medical soft robotics to mathematical analysis, Wellmann locates biological motion at the intersection of knowledge domains and scientific and cultural practices. She offers signposts to mark the sites where researchers, technologies, ideas, and practices opened up new paths in the constitution of the phenomenon of motion. An ambitious rethinking of the life sciences, Biological Motion uncovers the secret life of movement and offers a new account of what it means to be alive.
Microrheology
Rheology is the study of the flow of matter. It is an important and active field of research that spans numerous disciplines and technological applications. The aim of this work is to provide an introduction to the theory and practice of microrheology, a relatively new area of rheology.
Stress-Strain Behavior of Single Vimentin Intermediate Filament
Cells are the basic unit of living organisms and consist of a cytoplasm, which is enclosed by a membrane. As building blocks of life with a plethora of functions, cells have to be equipped with a high degree of mechanical resistance, durability, and variability. In eukaryotic cells three filamentous protein types – actin filaments, microtubules, and intermediate filaments (IFs) – form the so-called cytoskeleton, a network that is known to play a key role for the mechanical properties of cells. Among the three filament systems, IFs are special in terms of, for example, their hierarchical architecture, and their cell-type specific expression. In this thesis, vimentin, an IF mostly found in...
