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Biotechnologies for Plant Mutation Breeding
This book is open access under a CC BY-NC 2.5 license. This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.
Jatropha, Challenges for a New Energy Crop
Jatropha curcas or Physic Nut is a small tree (bush plant) that produces fruits under tropical climate. The fruits contained seed that are ~40% oil rich. This oil is excellent for biodiesel. The bush is a now new coming crop because it may cope with harsh environmental conditions such as semi-aridity and poor land. It is considered as one alternative for climate mitigation that does not compete with arable land normally dedicated to food crop and can be used to regain degraded land or fight desertification. This bush has been considered seriously by the international community only recently (~2006-2008), but worldwide scientists did an outstanding job to drawn Jatropha out of its semi-wild s...
The Jatropha Genome
This book presents the genetics and genomics of Jatropha, which is used for biofuel, and shows how plant genomics can be used to improve plant breeding. The utilization of plant biofuels is a promising solution to global issues such as the depletion of fossil fuels and resources and climate change. Jatropha curcas L. (jatropha) is a species of shrub belonging to the Euphorbiaceae family. Native to Mesoamerica, it is now grown widely in tropical and subtropical areas in America, Africa and Asia. The seed oil of Jatropha is a suitable source for biodiesel or bio jet fuel, and since it is not edible and can grow in semi-arid lands unsuitable for the cultivation of food crops, its production does not compete with that of food to inflate its price. The characteristics of this promising biofuel plant, however, have not been fully exploited in terms of breeding, mainly because of the lack of information on its genetics and genomics. The structure of the whole genome of Jatropha is analyzed, providing insights into on the plant’s genetic system and accelerating the molecular breeding process.
Applied Plant Genomics and Biotechnology
Applied plant genomics and biotechnology reviews the recent advancements in the post-genomic era, discussing how different varieties respond to abiotic and biotic stresses, investigating epigenetic modifications and epigenetic memory through analysis of DNA methylation states, applicative uses of RNA silencing and RNA interference in plant physiology and in experimental transgenics, and plants modified to produce high-value pharmaceutical proteins. The book provides an overview of research advances in application of RNA silencing and RNA interference, through Virus-based transient gene expression systems, Virus induced gene complementation (VIGC), Virus induced gene silencing (Sir VIGS, Mr V...
From Plant Genomics to Plant Biotechnology
With the appearance of methods for the sequencing of genomes and less expensive next generation sequencing methods, we face rapid advancements of the -omics technologies and plant biology studies: reverse and forward genetics, functional genomics, transcriptomics, proteomics, metabolomics, the movement at distance of effectors and structural biology. From plant genomics to plant biotechnology reviews the recent advancements in the post-genomic era, discussing how different varieties respond to abiotic and biotic stresses, understanding the epigenetic control and epigenetic memory, the roles of non-coding RNAs, applicative uses of RNA silencing and RNA interference in plant physiology and in ...
