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Cardiovascular Proteomics
This cutting-edge book presents protocols and strategies for proteomic evaluation of cardiovascular disease written by pioneering researchers in the field. Topics explored in this comprehensive volume include obtaining specific heart proteins, techniques for identifying risk biomarkers of atherome plaque rupture, analyzing the secretome of explanted endarterectomies cultured in vitro, and phage display techniques for deciphering the molecular diversity of blood vessels.
Platelet Proteomics
The purpose of the book is to introduce platelets, and their functional role in thrombotic and cardiovascular disease, justifying the relevance of platelet proteomics research. Focus then shifts to the recent developments on mass spectrometry (MS)-based proteomics. This chapter shows potential applications for platelet proteomics not yet carried out. It includes examples of post-translational modifications (PTMs) analysis in platelets. The second part of the book focuses on the main research done so far on platelet proteomics. This includes general proteome mapping by non-gel based separation methods (MudPit), analysis of the general platelet proteome and signaling cascades by gel-based separation methods (2-DE), sub-proteome analyses (secretome/releasate, membrane proteins, organelles). Finally, the last section links the platelet transcriptome and application to disease. This section is highly relevant and includes chapters on proteomics, transcriptomics, functional genomics, systems biology, and their applications to platelet-related diseases.
Applications of Advanced Omics Technologies: From Genes to Metabolites
The book contains contributions concerning the application of the new instrumental and methodological developments in omics technologies, including those related to Genomics, Transcriptomics, Proteomics, Peptidomics and Metabolomics, Lipidomics and Foodomics. The16 chapters discuss in detail: innovative applications of functional gene microarrays for profiling microbial communities, microRNA profiling, novel genotyping applications using microarray technology in cancer research, next-generation sequencing applied to the study of human microbiome, emerging RNA-SEQ applications in food science, recent progress in plant proteomics, applications of gel-free proteomic approaches, the challenges a...
The Public Relations Practitioner's Playbook
"The Public Relations Practitioner's Playbook" is how-to and hands-on. Theory is woven into thousands of proven techniques, tips, tactics, tools and strategies spread over nearly 600 pages. Explanations, examples and anecdotes are in a language that should appeal to experienced practitioners, college students and organization volunteers who assist with public relations and publicity. "The PR Practitioner's Playbook" - an anatomy of the public relations profession - relies on my experience as a reporter, editor, public relations counselor, and strategic advisor and evaluator. It demonstrates that successful writers practice their craft with poise and eloquence. It is an extension of the autho...
High Content Screening
There has always been some tension between proponents of hypothesis-driven and discovery-driven research in the broad field of life sciences. Academic research has been primarily focused on hypothesis-driven research. However, the success of the human genome project, a discovery-driven research approach, has opened the door to adding other types of discovery-driven research to a continuum of research approaches. In contrast, drug discovery research in the pharmaceutical industry has embraced discovery-driven research for many years. A good example has been the discovery of active compounds from large chemical libraries, through screening campaigns. The success of the human genome project has...
New Insights in Anaphylaxis
Anaphylaxis has usually been studied and understood from an immunological view and the main lethal effects involve the cardio-vascular system. Despite exhaustive efforts exerted in this area of research, much more investigation is needed to mitigate, recognize or prevent this disease. The scope of this Research Topic is to present and discuss the current knowledge of anaphylaxis, from the clinic to the underlying molecular mechanisms. We are thankful to the medical doctors, researchers, and colleagues who have contributed to this issue, as it would not have been possible without their highly valuable expertise in allergy and anaphylaxis research.
The Public Relations Practitioner's Playbook for (all) Strategic Communicators
Some years ago, a young graduate student contributed to a book for educational public relations specialists. It was a “how-to-do-it book, light on theory and without footnotes” that offered hundreds of tips and “ideas.” Its title evolved into School Communication Ideas that Work. Like that successful and widely used book, published in 1972, The Public Relations Practitioner's Playbook for (all) Strategic Communicators is how-to and hands-on. Edition three was considered for the 2009 Pulitzer Prize. The theory it contains is woven into thousands of proven techniques, tips, tactics, tools and strategies spread over 626 pages. Explanations, examples and anecdotes are in a language that ...
Proteomics of Microbial Human Pathogens
According to the World Health Organization (WHO), in 2012 infectious diseases and related conditions account for more than 70% of premature deaths across 22 African countries and estimated 450 000 people worldwide developed multi-drug resistant tuberculosis. This alarming situation, of great public health concern, calls for the urgent development of novel and efficient responding strategies. The employment of important research platforms, such as genomics and proteomics, has contributed significant insight into the mechanisms underlying microbial infection and microbe-host interaction. In this Frontiers Research Topic, we aim to produce a timely and pertinent discussion regarding the current...
Pedro Almodóvar
Perhaps the best-known Spanish filmmaker to international audiences, Pedro Almodóvar gained the widespread attention of English-speaking critics and fans with the Oscar-nominated Women on the Verge of a Nervous Breakdown and the celebrated dark comedy Tie Me Up! Tie Me Down!. Marvin D'Lugo offers a concise, informed, and insightful commentary on a preeminent force in modern cinema. D'Lugo follows Almodóvar's career chronologically, tracing the director's works and their increasing complexity in terms of theme and the Spanish film tradition. Drawing on a wide range of critical sources, D'Lugo explores Almodóvar's use of melodrama and Hollywood genre film, his self-invention as a filmmaker,...
Desire Unlimited
The huge international success of his latest feature, All About My Mother, has finally granted Pedro Almodovar the recognition he deserves, as the most artistically ambitious and commercially consistent film-maker in Europe.
