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This volume discusses traditional and current techniques that are successfully used to diagnose plant viruses and study molecular plant-virus interactions. The chapters in this book cover topics such as in vivo detection of double-stranded RNA, developing rice mutant using CRISPR-Cas9-based technology, protein-protein interaction assays, purification and transfection of protoplasts, protocols for gene silencing, and transmission electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Plant Virology: Methods and Protocols is a valuable resource for plant pathologists, microbiologists, virologists, graduate students, and teachers who are interested in learning more about the developments in plant virology research.
e would like to welcome you to the ASIAN CONFERENCE ON HUMANITIES, INDUSTRY, AND TECHNOLOGY FOR SOCIETY hosted by, Dr Soetomo University on 30 - 31 July 2019 at Dr Soetomo University, Surabaya, East Java, Indonesia. The conference aims to provide all researchers with the opportunity to share their research in the areas of Social Science, Industry, & Technology to the International community. This Conference accepts all paper related to Humanities, Industrial Revolution, Applied Technology and Engineering for Sustainable Society and our Objectives is to promote an exchange of research ideas and knowledge among local and international researchers and alsi to provide a platform for research collaborations among local and international researchers and institutions of higher learning.
The precarious equilibrium among the four Londons has reached its breaking point. Once brimming with the red vivacity of magic, darkness casts a shadow over the Maresh Empire, leaving a space for another London to rise. Kell begins to waver under the pressure of competing loyalties. Meanwhile, an ancient enemy returns to claim a crown and a fallen hero is desperate to save a decaying world...
"Alif had never set foot outside of West Sumatra. He passed his childhood days searching for fallen durian fruit in the jungle, playing soccer on rice paddies, and swimming in the blue waters of Lake Maninjau. His mother wants him to attend an Islamic boarding school, a pesantren, while he dreams of public high school. Halfheartedly, he follows his mother’s wishes. He finds himself on a grueling three-day bus ride from Sumatra to Madani Pesantren (MP) in a remote village on Java. On his first day at MP, Alif is captivated by the powerful phrase man jadda wajada. He who gives his all will surely succeed. United by punishment, he quickly becomes friends with five boys from across the archipe...
Lonesome Lily turned Scandalous Siren Miss Lillian Hargrove has lived much of her life alone in a gilded cage, longing for love and companionship. When an artist offers her pretty promises and begs her to pose for a scandalous portrait, Lily doesn’t hesitate . . . until the lying libertine leaves her in disgrace. With the painting now public, Lily has no choice but to turn to the one man who might save her from ruin. Highland Devil turned Halfhearted Duke The Duke of Warnick loathes all things English, none more so than the aristocracy. It does not matter that the imposing Scotsman has inherited one of the most venerable dukedoms in Britain—he wants nothing to do with it, especially when he discovers that the unwanted title comes with a troublesome ward, one who is far too old and far too beautiful to be his problem. Tartan Comes to Town Warnick arrives in London with a single goal: get the chit married and see her become someone else’s problem, then return to a normal, quiet life in Scotland. It’s the perfect plan, until Lily declares she’ll only marry for love . . . and the Scot finds that there is one thing in England he likes far too much . . .