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Fluorescent Probes
The photophysical and photochemical properties of probes in biological and in model systems are topics of intense current research. The wavelength, intensity, time and polarisation dependence of fluorescence probes can provide information on properties as diverse as electric fields and changes in conformation and may be used in the formidable task of establishing structure-function relationships in proteins and cell membranes. The wide scope of interactions which can be studied by these fluorescent molecules is illustrated in the articles in this volume which are based on talks, although much extended and updated, given at a meeting on 'Fluorescence Probes in Proteins and Membranes' held in the Royal Institution in November 1979.
Fluorescent and Luminescent Probes for Biological Activity
The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes.The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy are also cov...
Fluorescence Microscopy and Fluorescent Probes
Fluorescence microscopy images can be easily integrated into current video and computer image processing systems. People like visual observation; they like to watch a television or computer screen, and fluorescence techniques are thus becoming more and more popular. Since true in vivo experiments are simple to perform, samples can be directly seen and there is always the possibility of manipulating the samples during the experiments; it is an ideal technique for biology and medicine. Images are obtained by a classical (now called wide-field) fluorescence microscope, a confocal scanning microscope, upright or inverted, with epifluorescence or transmission. Computerized image processing may improve definition, and remove glare and scattered light signal. It also makes it possible to compute ratio images (ratio imaging both in excitation and in emission) or lifetime imaging. Image analysis programs may supply a great deal of additional data of various types, starting with calculations of the number of fluorescent objects, their shapes, brightness, etc. Fluorescence microscopy data may be complemented by classical measurement in the cuvette yr by flow cytometry.
Handbook of Fluorescent Dyes and Probes
A COMPLETE, UP-TO-DATE RESOURCE OF INFORMATION ON MORE THAN 150 FLUORESCENT DYES AND PROBES Handbook of Fluorescent Dyes and Probes is the most comprehensive volume available on the subject, covering all the available dyes and probes known to date in the literature for uses in various fields. Top dye expert Dr. Ram Sabnis organizes the compounds alphabetically by the most commonly used chemical name. He presents an easy-to-use reference complete with novel ideas for breakthrough research in medical, biological, chemical, color, material, physical and related allied fields. The ease of use of the handbook is further enhanced by various appendixes provided at the end of the book to convenientl...
Fluorescent Energy Transfer Nucleic Acid Probes
Fluorescent nucleic acid probes, which use energy transfer, include such constructs as molecular beacons, molecular break lights, Scorpion primers, TaqMan probes, and others. These probes signal detection of their targets by changing either the intensity or the color of their fluorescence. Not surpr- ingly, these luminous, multicolored probes carry more flashy names than their counterparts in the other fields of molecular biology. In recent years, fluor- cent probes and assays, which make use of energy transfer, have multiplied at a high rate and have found numerous applications. However, in spite of this explosive growth in the field, there are no manuals summarizing different p- tocols and...
Analytical Use of Fluorescent Probes in Oncology
Fluorescence is a very powerful tool for work at the frontier of cell biology, photobiology and bioinstrumentation. The stated aim of the workshop was to highlight the significance of fluorescence work for the understanding of cell and tissue physiology, physiopathology and pharmacology, particulary in terms of the analytical use of fluorescent probes in oncology. In the organization of the workshop a multidisciplinary approach was selected. The purpose of the Advanced Research Workshop (ARW) was to bring together researchers in the various disciplines of tissue optics, imaging, microspectrofluorometry and state of the art probes, in order to explore the full benefits that can be derived in ...
Fluorescence Microscopy and Fluorescent Probes
The April 1997 conference held in Prague attracted the cream of primarily European and Russian researchers (with a handful from the US, primarily from the U. of Maryland School of Medicine) to the burgeoning biological and medical applications of innovative optical technology, particularly laser confocal fluorescence microscopy. Six invited review lectures illuminate these recent developments and their role in cell biology, including computer simulations of molecular behavior. The 34 other papers focus on the major themes of: new developments in fluorescence instrumentation; fluorescent probes; nucleic acid labels; and other fluorescent labels, markers, and fluorogenic substrates. Annotation copyrighted by Book News, Inc., Portland, OR
Fluorescent Probes and Sensors
This book is a printed edition of the Special Issue "Fluorescent Probes and Sensors" that was published in Sensors
Fluorescent Probes in Cellular and Molecular Biology
Fluorescent Probes in Cellular and Molecular Biology presents the most comprehensive evaluation of the theory, instrumentation, and practical applications of fluorescent probes available. It covers basic principles and discusses the use of fluorescent dyes as probes for solvent polarity, solvent viscosity, membrane fluidity, membrane potential, and ionic intracellular composition. This important also describes various applications of fluorescent labels and photolabels, fluorescent analogs, and fluorogenic substrates. No similar publication is devoted exclusively to fluorescent probes, making this an essential guide for anyone working with modern fluorescence techniques. The book is perfect for individuals working in the areas of photochemistry, photobiology, biology, biophysics, or biochemistry. It is also an excellent textbook for graduate students in biology, biophysics, and medicine.
