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Still widely used as gene markers, isozymes detected by zymogram techniques have proven valuable in a range of other biological applications over the last few years. Along with these new applications, many new techniques have also emerged. Yet more than eight years since the Handbook of Detection of Enzymes on Electrophoretic Gels was first publish
Discussing all aspects connected with the scientific analysis of Rhipicephalus microplus, this book covers tick classification and identification, as well as methods of extracting natural products effective against ticks. It also describes tick cell culture procedures, tick acaricide-resistance diagnostics, and the identification of tick parasites and microorganisms from the host and the ticks’ fluids, as well as the diagnosis of Babesia and Anaplasma in R. microplus.
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This detailed volume examines the latest techniques and protocols associated with zymography, the practice of detecting enzyme activity on electrophoretic gels. Sections cover subjects such as endopeptidase zymography, reverse zymography & in situ zymography, 2D zymography, as well as a variety of special cases. Written in the highly respected Methods in Molecular Biology series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Zymography: Methods and Protocols serves as an ideal aid for a broad array of researchers from the fields of biological and medicinal science, as well as scientists working in clinical and diagnostic medicine, medical genetics, agricultural entomology, genetic monitoring of environmental pollution, and forensic science.
In 1966 Congress passed the National Sea Grant College Program Act to promote marine research, education, and extension services in institutions along the nation's ocean and Great Lakes coasts. In Maryland a Sea Grant Program -- a partnership among federal and state governments, universities, and industries -- began in 1977, and in 1982 the University of Maryland was named the nation's seventeenth Sea Grant College. The Maryland Sea Grant College focuses its efforts on the Chesapeake Bay, with emphasis on the marine concerns of fisheries, seafood technology, and environmental quality. The first comprehensive review of the biology of the eastern oyster in more than thirty years. The twenty-one chapters synthesize every aspect of oyster biology -- for instance, general anatomy, physiology, the circulatory system, reproduction, genetics, diseases -- and issues related to management and aquaculture.