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Essential Zebrafish Methods: Genetics and Genomics
Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues that make it the model organism for study of vertebrate development.This book, one of two new volumes in the Reliable Lab Solutions series dealing with zebrafish, brings together a robust and up-to-date collection of time-tested methods presented by the world's leading scientists. Culled from previously published chapters in Methods in Cell Biology and updated by the original authors where rel...
The Zebrafish: Disease Models and Chemical Screens
This volume of Methods in Cell Biology is the 3e, and provides comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This new volume on Disease Models and Chemical Screens, covers two rapidly emerging and compelling applications of the zebrafish. - Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls - This volume concentrates on Disease Models and Chemical Screens
The Zebrafish
The zebrafish (Danio rerio) is a valuable and common model for researchers working in the fields of genetics, oncology and developmental sciences. This full-color atlas will aid experimental design and interpretation in these areas by providing a fundamental understanding of zebrafish anatomy. Over 150 photomicrographs are included and can be used for direct comparison with histological slides, allowing quick and accurate identification of the anatomic structures of interest. Hematoxylin and eosin stained longitudinal and transverse sections demonstrate gross anatomic relationships and illustrate the microscopic anatomy of major organs. Unlike much of the current literature, this book is focused exclusively on the zebrafish, eliminating the need for researchers to exclude structures that are only found in other fish.
The Zebrafish: Genetics and Genomics
This is the second volume of a two-volume, comprehensive treatment of the methodologies used in researching the zebrafish, an emerging vertebrate model system. The text includes discussions on development, genetic methodologies, and model applications.Key Features* Details state-of-the-art zebrafish protocols in a single-source reference* Presents methods and reagents in user-friendly format* Delineates critical steps and pitfalls of he procedures* Illustrates techniques with full-color plates* Summarizes many new and interesting developmental mutants* Includes appendices with strain information and a compendium of zebrafish World Wide Web sites* Relevant to clinicians interested in vertebrate models of human congenital diseases
The Antarctic Silverfish: a Keystone Species in a Changing Ecosystem
This book encompasses the body of available scientific information on the notothenioid fish Pleuragramma antarctica commonly known as Antarctic silverfish. This plankton-feeder of the intermediate trophic level is the most abundant fish in the coastal regions of high Antarctica, and plays a pivotal ecological role as the main prey of top predators like seals, penguins, whales and Antarctic toothfish. Broad circum-polar distribution, a key role in the Antarctic shelf pelagic ecosystem, and adaptations makes understanding the species’ likely response to environmental change relevant to foresee the potential responses at the local ecosystem level. Additionally, a detailed understanding of the...
Antarctic Ecosystems
Since its discovery Antarctica has held a deep fascination for biologists. Extreme environmental conditions, seasonality and isolation have lead to some of the most striking examples of natural selection and adaptation on Earth. Paradoxically, some of these adaptations may pose constraints on the ability of the Antarctic biota to respond to climate change. Parts of Antarctica are showing some of the largest changes in temperature and other environmental conditions in the world. In this volume, published in association with the Royal Society, leading polar scientists present a synthesis of the latest research on the biological systems in Antarctica, covering organisms from microbes to vertebrate higher predators. This book comes at a time when new technologies and approaches allow the implications of climate change and other direct human impacts on Antarctica to be viewed at a range of scales; across entire regions, whole ecosystems and down to the level of species and variation within their genomes. Chapters address both Antarctic terrestrial and marine ecosystems, and the scientific and management challenges of the future are explored.
Neurons: Methods and Applications for the Cell Biologist
Neurons: Methods and Applications for the Cell Biologist lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjects include peripheral and central neurons from vertebrate and invertebrate sources, as well as neuron-like cell lines. It also explains recent advances in our ability to introduce exogenous proteins and genes to neurons in culture. Procedures for successful protein infiltration, biolistic transfection, electroporation, and viral transgenic methods in neurons are also presented. - Contains culture methodology for more than a dozen types of CNS and PNS neurons - Includes most recent and reliable techniques from expert practitioners for specific experimental applications - Addresses the latest strategies for transfecting neurons
Biophysical Tools for Biologists
Driven in part by the development of genomics, proteomics, and bioinformatics as new disciplines, there has been a tremendous resurgence of interest in physical methods to investigate macromolecular structure and function in the context of living cells. This volume in Methods in Cell Biology is devoted to biophysical techniques in vivo and their applications to cellular biology. Biophysical Tools for Biologists covers methods-oriented chapters on fundamental as well as cutting-edge techniques in molecular and cellular biophysics. This book is directed toward the broad audience of cell biologists, biophysicists, pharmacologists, and molecular biologists who employ classical and modern biophysical technologies or wish to expand their expertise to include such approaches. It will also interest the biomedical and biotechnology communities for biophysical characterization of drug formulations prior to FDA approval. - Describes techniques in the context of important biological problems - Delineates critical steps and potential pitfalls for each method
Methods in Cell-Matrix Adhesion
Critically acclaimed for more than 25 years, the Methods in Cell Biology series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made accessible to users. This volume, Methods of Cell-Matrix Adhesion, contains integrated coverage on cell-matrix adhesion methods. It brings the classical methodologies and the latest techniques together in one concise volume. This coverage includes experimental protocols and their conceptual background for all aspects of cell-matrix adhesion research: the extracellular matrix, adhesion receptors, and the growing number of functional applications of matrix-adhesion in molecular cell biology. Also covered is the purification of the extracellular matrix to functional analyses of cellular responses.
Stem Cell Culture
The purpose of Stem Cell Culture is to provide a comprehensive resource for researchers in the fields of embyonic, fetal and adult stem cell biology to find methods for the purification, culture, and differentitation of these cell types, with the main emphasis on the maintenance of the stem cell phenotype in vitro. This volume will be the first to broadly cover multiple types of stem cell culture from different ages, organs and species. Authors will focus on the practical do's and don'ts of isolating and culturing these cell types, and feel free to use illustrative data or diagrams whereever this improves the comprehension of the reader. This should allow the reader to compare and contrast techniques and make this a standard reference for those in the field, or desiring to start stem cell culture. - Describes techniques in stem cell research - Delineates critical steps and potential pitfalls for each method - Covers specific procedures in dealing with Human Embryonic Stem Cells
