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The Hepatocyte Review
It is thirty years since the technique of high-yield preparation of isolated hepatocytes, by collagenase perfusion of the liver, was published. The original method described by Berry and Friend has undergone many minor modifications by other workers, and the two-step procedure introduced by Seglen in 1976 has become the most frequent way to prepare hepatocyte suspensions. An important development introduced by Bissell in 1973 was the use of the cells as the first step in monolayer culture. The availability of the isolated hepatocyte preparation as cells in suspension or culture has undoubtedly facilitated research on the liver. This was emphasised in our book, published (with Dr. Greg Barrit...
Animal Cell Technology: Basic & Applied Aspects
Animal cell technology has been making tremendous progress. Originally this term reminded people of engineering for high density and large volume culture of animal cells. At present many fields of biological sciences are aiming at advance in animal cell technology. Cell culture engineering is aided not only with developments in apparatus, matrix, media, and computational analysis, but also with new biological procedures in gene and protein technology, cell biological resources and immunological methods. Results obtained with animal cell technology are applied to production of pharmaceuticals, diagnosis reagents and food endowed with physiological functions, and cell and gene therapy of anima...
Arthropod Venom Components and Their Potential Usage
Thousands of arthropod species, ranging from arachnids (spiders and scorpions) to hymenopterans (ants, bees, and wasps) and myriapods (centipedes), are venomous and use their venoms for both defense and predation. These venoms are invariably harmful to humans, and some may cause serious injuries, e.g., those from scorpions, spiders, and wasps. Arthropods’ venoms are also known as rich sources of biologically active compounds and have attracted the attention of toxin researchers for years. In this century, venom component analysis has progressed considerable due to the advances in analytical techniques, in particular, mass spectrometry and next-generation deep (DNA and RNA) sequencing. As s...
Material and Manufacturing Technology VII
Selected, peer reviewed papers from the 2016 7th International Conference on Material and Manufacturing Technology, May 14-16, 2016, Chiang Mai, Thailand
Tsunamis: Their Science and Engineering
Proceedings of the International Tsunami Symposium 1981, IUGG Tsunami Commission, May, 1981, Sendai-Ofunato-Kamaishi, Japan
