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Activation and Suppression of Plant Immunity
Plants constantly face many kinds of abiotic and biotic stresses. One of the major threats is from many plant fungal, oomycete, viral, bacterial and nematode pathogens. Plant diseases caused by these pathogens reduce crop yield by 10-15% worldwide every year. Throughout the human history, plant diseases are responsible for many famines including the infamous Irish Potato Famine. Besides the negative impact on the yield, the quality of the infected crop will be adversely affected and the toxins produced by plant pathogens pose threat to human health. During the co-evolution between plants and pathogens, plants developed elegant defense system against pathogen infection and plant pathogens deploy a variety of strategies to suppress plant innate immunity. A deeper understanding the molecular mechanisms on the activation of plant defense in plants and suppression of plant defense by plant pathogens will be crucial to develop effective ways to minimize the detrimental effects from plant diseases on human beings. This Research Topic aims to increase our understanding on the molecular interactions between plants and pathogens.
Base Editors
This volume explores base editors (BEs), an invaluable CRISPR-based genome editing tool with a wide variety of versatile applications. Beginning with an overview of BEs, their diverse variants, and computational tools, the book continues with experimental applications of BEs for disease modeling in mammalian cells and generating mutagenic mice, therapeutic base editing strategies, which covers delivery methods of BE-encoded DNA plasmids, mRNAs, or ribonucleoproteins through viruses or non-viral lipid nanoparticles, and lastly, the use of BEs in plants and bacteria. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Base Editors: Methods and Protocols serves as an ideal guide for researchers looking to use base editors to continue their studies in an array of fields.
Self-Spreading Biotechnology and International Law
Wer haftet, wenn sich selbst ausbreitende Gentechnik grenzüberschreitende Schäden verursacht? Mit Gene Drives und ähnlichen Verfahren wird es bald möglich sein, das Erbgut wild lebender Arten, Keime und Nutzpflanzen direkt in der Umwelt zu verändern. Dies könnte helfen, drängende Probleme in der öffentlichen Gesundheit, im Naturschutz und in der Ernährungssicherheit zu lösen. Allerdings bergen diese Verfahren auch das Risiko einer unkontrollierten Ausbreitung über Staatsgrenzen hinweg. Anhand einer grundlegenden Untersuchung der einschlägigen Verträge und des Völkergewohnheitsrechts zu Prävention und Haftung für grenzüberschreitende Schäden wird aufgezeigt, dass das derzeit geltende Völkerrecht dieser Herausforderung noch nicht gewachsen ist.
Global Regulatory Outlook for CRISPRized Plants
Global Regulatory Outlook of CRISPRized Plants summarizes CRISPR/Cas systems and applications for precise editing of plant genomes and discusses the global regulatory framework for CRISPR edited crops. While CRISPR technology has become a routine, cheap and an efficient method to generate edited crops with superior traits, how these crops will be regulated, will determine the future of this technology. Understanding the current state of regulation, the concerns, issues and foundations for decisions will be key in determining how this technology is used going forward. Global Regulatory Outlook of CRISPRized Plants highlights regulatory classification of CRISPR modifications such as SDN1, SDN2...
