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Antibody Engineering
The exquisite binding specificity of antibodies has made them valuable tools from the laboratory to the clinic. Since the description of the murine hybridoma technology by Köhler and Milstein in 1975, a phenomenal number of mo- clonal antibodies have been generated against a diverse array of targets. Some of these have become indispensable reagents in biomedical research, while others were developed for novel therapeutic applications. The attractiveness of an- bodies in this regard is obvious—high target specificity, adaptability to a wide range of disease states, and the potential ability to direct the host’s immune s- tem for a therapeutic response. The initial excitement in finding P...
Environmental Biotechnology
The past 30 years have seen the emergence of a growing desire worldwide that positive actions be taken to restore and protect the environment from the degrading effects of all forms of pollution – air, water, soil, and noise. Since pollution is a direct or indirect consequence of waste production, the seemingly idealistic demand for “zero discharge” can be construed as an unrealistic demand for zero waste. However, as long as waste continues to exist, we can only attempt to abate the subsequent pollution by converting it to a less noxious form. Three major questions usually arise when a particular type of pollution has been identi ed: (1) How serious is the pollution? (2) Is the techno...
Environmental and Natural Resources Engineering
This volume has been designed to serve as a natural resources engineering reference book as well as a supplemental textbook. This volume is part of the Handbook of Environmental Engineering series, an incredible collection of methodologies that study the effects of resources and wastes in their three basic forms: gas, solid, and liquid. It complements two other books in the series including "Natural Resources and Control Processes" and "Advances in Natural Resources Management". Together they serve as a basis for advanced study or specialized investigation of the theory and analysis of various natural resources systems. This book covers many aspects of resources conservation, treatment, recy...
Genetics Manual
"Redei has created an outstanding compendium of genetics. Arranged as a dictionary, the book is almost an encyclopedic collection of terms & concepts ... The author has managed to define terms with appropriate mixtures of depth & detail for the researcher, along with clarity useful for the nonexpert." Choice, 1998
Movement Disorders
The use of animal models is a key aspect of scientific research in numerous fields of medicine. This book vigorously examines the important contributions and application of animal models to the understanding of human movement disorders and will serve as an essential resource for basic neuroscientists engaged in movement disorders research. Academic clinicians, translational researchers and basic scientists are brought together to connect experimental findings made in different animal models to the clinical features, pathophysiology and treatment of human movement disorders. A vital feature of this book is an accompanying DVD with video clips of human movement disorders and their correspondin...
Protein Structure, Stability, and Folding
In Protein Structure, Stability, and Folding, Kenneth P. Murphy and a panel of internationally recognized investigators describe some of the newest experimental and theoretical methods for investigating these critical events and processes. Among the techniques discussed are the many methods for calculating many of protein stability and dynamics from knowledge of the structure, and for performing molecular dynamics simulations of protein unfolding. New experimental approaches presented include the use of co-solvents, novel applications of hydrogen exchange techniques, temperature-jump methods for looking at folding events, and new strategies for mutagenesis experiments. Unique in its powerful combination of theory and practice, Protein Structure, Stability, and Folding offers protein and biophysical chemists the means to gain a more comprehensive understanding of some of this complex area by detailing many of the major techniques in use today.
Pesticide Protocols
A comprehensive collection of robust methods for the detection of pesticide compounds or their metabolites useful in food, environmental, and biological monitoring, and in studies of exposure via food, water, air, and the skin or lungs. The readily reproducible methods range from gas and liquid chromatography coupled to mass spectrometry detection and other classic detectors, to capillary electrophoresis and immunochemical or radioimmunoassay methods. The authors have focused on extraction and cleanup procedures, in order to develop and optimize more fullyautomated and miniaturized methods, including solid-phase extraction, solid-phase microextraction, microwave-assisted extraction, and on-line tandem liquid chromatography (LC/LC) trace enrichment, among others. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Nutrition and Infectious Diseases
This comprehensive and user-friendly volume focuses on the intersection between the fields of nutrition and infectious disease. It highlights the importance of nutritional status in infectious disease outcomes, and the need to recognize the role that nutrition plays in altering the risk of exposure and susceptibility to infection, the severity of the disease, and the effectiveness of treatment. Split into four parts, section one begins with a conceptual model linking nutritional status and infectious diseases, followed by primers on nutrition and immune function, that can serve as resources for students, researchers and practitioners. Section two provides accessible overviews of major catego...
Cytokines and Colony Stimulating Factors
The immune system is a complex network in which different cell types and soluble factors interact to efficiently eliminate various kinds of microorganisms as well as aberrant cell clones. The roots of immunologic investigations reach far into the past. In 430 BC, Thucydides reported that survivors of the plague did not present a second time with similar symptoms. The first report of a successful immu- therapy was made by Edward Jenner in 1798 who found a protective effect of cowpox vaccination against human pox. Since then, much knowledge has been accumulated; today, investigations of the molecular mechanisms of immune regulation are of central research interest. The novel insights into gene...
Recombinant Gene Expression
Since newly created beings are often perceived as either wholly good or bad, the genetic alteration of living cells impacts directly on a symbolic meaning deeply imbedded in every culture. During the earlier years of gene expression research, te- nological applications were confined mainly to academic and industrial laboratories, and were perceived as highly beneficial since molecules that were previously unable to be separated or synthesized became accessible as therapeutic agents. Such were the success stories of hormones, antibodies, and vaccines produced in the bacterium Escherichia coli. Originally this bacterium gained fame among humans for being an unwanted host in the intestine, or w...
