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Fission Yeast
Fission yeast are unicellular, rod-shaped fungi that divide by medial fission. Studies using fission yeast were instrumental in identifying fundamental mechanisms that govern cell division, differentiation, and epigenetics, to name but a few. Their rapid growth rate, genetic malleability, and similarities to more complex eukaryotes continue to make them excellent subjects for many biochemical, molecular, and cell biological studies. This laboratory manual provides an authoritative collection of core experimental procedures that underpin modern fission yeast research. The contributors describe basic methods for culturing and genetically manipulating fission yeast, synchronization strategies f...
Fission Yeast
Fission yeast are unicellular, rod-shaped fungi that divide by medial fission. Studies using fission yeast were instrumental in identifying fundamental mechanisms that govern cell division, differentiation, and epigenetics, to name but a few. Their rapid growth rate, genetic malleability, and similarities to more complex eukaryotes continue to make them excellent subjects for many biochemical, molecular, and cell biological studies. This laboratory manual provides an authoritative collection of core experimental procedures that underpin modern fission yeast research. The contributors describe basic methods for culturing and genetically manipulating fission yeast, synchronization strategies f...
Introduction to Electron Microscopy for Biologists
This volume demonstrates how cellular and associated electron microscopy contributes to knowledge about biological structural information, primarily at the nanometer level. It presents how EM approaches complement both conventional structural biology (at the high end, angstrom level of resolution) and digital light microscopy (at the low end, 100-200 nanometers). Basic techniques in transmission and scanning electron microscopy Detailed chapters on how to use electron microscopy when dealing with specific cellular structures, such as the nucleus, cell membrane, and cytoskeleton Discussion on electron microscopy of viruses and virus-cell interactions
The Molecular Biology of Schizosaccharomyces pombe
The fission yeast Schizosaccharomyces pombe is the favoured tool of many productive research groups throughout the world, serving as a useful model for fundamental principles and mechanisms, such as genome organization, differential gene regulation, cell-cycle control, signal transduction, or cellular morphogenesis. This book collates the current state of knowledge derived from molecular studies in this simple eukaryotic microorganism. The entire sequence of its genome has been completed, emphasizing the comparative value and model status of this yeast. The individual chapters, highlighting up-to-date views on prominent aspects of molecular organization, were written by active research scientists, presenting the results of their investigations to other workers in neighbouring fields. This book intends to serve the fission yeast community as a handy source of reference for years to come. It will also be of particular value to the ever-increasing number of researchers starting to look into fission yeast affairs for comparative reasons from other platforms of molecular genetics and cell biology.
The Centrosome in Cell Replication and Early Development
Centrosomes play an integral role in the growth of cells and the ultimate development of many animals, and sometimes plants. In addition to the normal growth of cells, centrosomes can also play a key role in the spread of cancer and are of increased interest to both the genetics and oncology communities. Volume 49 of Current Topics in Developmental Biology will present all known research surrounding the centrosome, across a variety of systems, will be well referenced, and speculate where the research is headed. - Discusses centrosomes and cancer, centrosomes and early development, and molecular biology of the centrosome - Heavily illustrated, with many color figures - Chapters written by international leaders in the field
Current Topics in Developmental Biology
Current Topics in Developmental Biology provides a comprehensive survey of the major topics in the field of developmental biology. The volumes are valuable to researchers in animal and plant development, as well as to students and professionals who want an introduction to cellular and molecular mechanisms of development. The series has recently passed its 30-year mark, making it the longest-running forum for contemporary issues in developmental biology. This volume contains nine important contributions from leading minds in developmental biology. - Presents major contemporary issues and astonishing discoveries at the forefront of modern developmental biology, stem cells, cloning, and regenerative medicine - Series Editor Gerald Schatten is one of the leading minds in reproductive and developmental science - The longest-running forum for current issues in developmental biology with over 30 years of coverage
Final FRCR 2B Viva
Final FRCR 2B Viva: A Survival Guide presents a series of cases similar to those used in the FRCR exams and representative of everyday radiological practice. This invaluable collection of high quality images is accompanied by clear and concise explanations, enabling trainees to prepare fully for their FRCR 2B viva presentation. Covering the full range of imaging modalities and organ systems, it provides clinically important vignettes which help the reader to impress examiners and colleagues, and enhance the trainee's ability to come up with differential diagnoses. Concise key points for each case provide additional diagnostic information which would impress an examiner. Written by a team of expert consultant radiologists and several recently successful FRCR Part 2 candidates, Final FRCR 2B Viva: A Survival Guide is an essential purchase for all radiology trainees.
Antibodies in Cell Biology
Antibodies in Cell Biology focuses on a new generation of protocols aimed at the cell biologist. This laboratory manual features systems and techniques that are especially relevant for modern problems. The contributing authors have been carefully chosen for their specific expertise, and have provided detailed protocols, recipes, and troubleshooting guides in each chapter. The book is designed for any researcher or student who needs to use antibodies in cell biology and related research areas.Practical applications and future emphases of antibodies, including: - Light microscopic immunolocalization of antigens - Gold particles in immunoelectron microscopy - Special methods of fixation and permeabilization - Microinjection of antibodies into living cells - Antibodies to identify cDNA clones - Antisense antibody strategies
Molecular Biology of the Cell
MBC online publishes papers that describe and interpret results of original research conserning the molecular aspects of cell structure and function.
Cell Biology E-Book
Reader-friendly Cell Biology, 4th Edition, provides a concise but comprehensive foundation for students entering research or health care career paths. Award winning illustrations help readers quickly grasp general principles. The authors have thoroughly updated this popular text to provide readers with the current understanding of the principles of normal cellular function along with examples of how molecular defects predispose to human disease. Major new themes in the 4th edition include the roles of intrinsically disordered polypeptides and phase separation in cellular functions, the influence of new molecular structures on understanding mechanisms, and the impact of exciting new methodsâ€...
