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Spectral and Imaging Cytometry
This second edition volume expands on the previous edition with discussions on the latest approaches used in the spectral cytometry field. Beginning with a brief history of spectral cytometry development, this book continues with a section addressing new protocols in different areas of imaging cytometry based on the unique technology of Imagestream and also introduces FlowCam – cytometer with capabilities of analyzing phytoplankton and zooplankton. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, complete lists of the materials and reagents necessary, and reproducible laboratory protocols with tips on troubleshooting and recommendations. Cutting-edge and thorough, Spectral and Imaging Flow Cytometry: Methods and Protocols, Second Edition is a valuable resources for beginners and professionals interested in learning more about this developing field. Chapters 1, 2, and 5 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Imaging Flow Cytometry
This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.
Image Analysis
The excellently received call for papers of the 13th Scandinavian Conference on Image Analysis, June 29-July 2 (SCIA 2003) resulted in the selected articles of this proceedings. Additionally the volume also contains invited contributions from - Ivar Austvoll, Stavanger University College (NO), - Lars B? a? ath, Halmstad University (SE), - Ewert Bengtsson, Uppsala University (SE), - Rasmus Larsen, Technical University of Denmark (DK), - Jussi Parkkinen, University of Joensuu (FI), - Pietro Perona, California Institute of Technology (US) which brings the total number of articles to 152. The theme of the papers are dominated by the categories - Feature extraction - Depth and surface - Medical image processing - Shape analysis - Segmentation and spatial grouping - Coding and representation - Motion analysis - Texture analysis - Color analysis - Indexing and categorization which also represent the topical groupings of this book. The particularly strong response to the feature extraction, depth and surface, and medical image processing themes makes us believe that these areas are c- rently expansive, partly because of the rich set of problems which remain to be addressed.
Cellular Heterogeneity
This volume is essential for anyone struggling with the cellular heterogeneity field, and it connects this new area with the latest advances in innovative techniques combined with practical advice from international experts. The chapters provide a comprehensive overview of cellular heterogeneity analysis techniques, exploring cytometry, imaging and spectroscopy methods, metabolic and molecular biological methods in tracking, as well as chromatin and cell cycle heterogeneity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Cellular Heterogeneity: Methods and Protocols encourages readers to explore new ways of studying cells that will help lead to exciting new discoveries.
Molecular Biology of the Cell
MBC online publishes papers that describe and interpret results of original research conserning the molecular aspects of cell structure and function.
The Challenge of New Therapeutic Approaches for Unmet Therapeutic Needs
This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.
Ecophysiology of Tropical Plants
Plants in tropical regions are coping with enormous challenges of physiological stresses owing to changing environmental and climatic conditions. Rapid growth of human population and rampant exploitation of fossil fuels and other developmental activities are actively contributing to such perturbations. The Intergovernmental Panel on Climate Change has projected a sustained increase in carbon dioxide (CO2) emissions and thereby a rise in global temperature in the coming decades. The resultant changes in precipitation patterns are now evident across the globe due to intensication of hydrological cycle. Moreover, gaseous and particulate pollutants are also an immense challenge for tropical plan...
