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This volume reviews the ecological effects of road, rail, marine and air transport. The focus ranges from identification of threats and repair of damaging effects to design of future transport systems that minimize environmental degradation. The scope of coverage extends from small ecosystems to the planet as a whole. Experts from a variety of disciplines address the topic, expressing views across the spectrum from deep pessimism to cautious optimism.
Electroporation is an efficient method to introduce macromolecules such as DNA into a wide variety of cells. Electrofusion results in the fusion of cells and can be used to produce genetic hybrids or hybridoma cells.Guide to Electroporation and Electrofusion is designed to serve the needs of students, experienced researchers, and newcomers to the field. It is a comprehensive manual that presents, in one source, up-to-date, easy-to-follow protocols necessary for efficient electroporation and electrofusion of bacteria, yeast, and plant and animal cells, as well as background information to help users optimize their results through comprehension of the principles behind these techniques. - Cove...
This book provides an up-to-date synthesis of the understanding of the interaction between the emission of nitrogen, its deposition and impact on the most important components of natural and semi-natural ecosystems. The work consists of contributions from internationally renowned research scientists. Individual chapters deal with the factors and processes related to nitrogen deposition and soils, non-forest vegetation communities, forest ecosystems, and surface waters. The assessment of these impacts is discussed in terms of setting critical loads. The book is aimed at researchers, advanced course students and policy makers/advisors involved with aspects of the impact of air pollution.
The goal of this book is to examine the complex state of radioactivity in the environment, and to understand the interplay of its two principal sources: man-made and natural. The text examines human contributions to release of radionuclides, with an eye to future reductions, and assesses natural occurrences in an evaluation of baseline radioactivity.
With millions of different bacterial species living in soil, the microbial community is extremely complex, varying at very small scales. Microbe-driven functions are essential for most processes in soil. Thus, a better understanding of this microbial diversity will be invaluable for the management of the various soil functions. Nucleic Acids and Proteins in Soil combines traditional approaches in soil microbiology and biochemistry with the latest techniques in molecular microbial ecology. Included are methods to analyse the presence and importance of nucleic acids and proteins both inside and outside microbial cells, the horizontal gene transfer which drives bacterial diversity, as well as soil proteomes. Further chapters describe techniques such as PCR, fingerprinting, the challenging use of gene arrays for structural and functional analysis, stable isotope probing to identify in situ metabolic functions, and the use of marker and reporter genes in soil microbial ecology.
Immunological Methods in Microbiology, Volume 47 in the Methods in Microbiology series, highlights new advances in the field, with this new volume presenting interesting chapters on Immunological Techniques in the Clinical laboratory, Immunologic Diagnosis of HIV and Opportunistic Infections, Combining Antigen Detection and Serology for the Diagnosis of Selected Infectious Diseases, Immunologic Detection of Lyme Disease and Related Borrelioses, Immunodetection of Bacteria Causing Brucellosis, Immunological Diagnostic Techniques Used to Identify and Type Pasteurella, Immunological Tests for Diarrhea caused by Diarrheagenic Escherichia coli Targeting Their Main Virulence Factors, and much more.
Biofilms, Volume 53 in the ongoing Methods in Microbiology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of timely topics, including Monospecies and polymicrobial biofilms in static and flow environment, Methods used to study biofilms, Spatial analysis of bacterial biofilms, Biofilm grown in bioreactors, Single-cell analysis of subpopulations within biofilms using microscopy, flow cytometry, and imaging flow cytometry, Microscopy analysis of biofilm-mineral interactions or mineral formation within biofilms, Bacterial biofilms as an essential component of rhizosphere plant-microbe interactions, Studying Gene Expression in Biofilms, and more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in Methods in Microbiology serials - Updated release includes the latest information on biofilms
This book is about the legal, economical, and practical assessment and management of risky activities arising from routine, catastrophic environmental and occupational exposures to hazardous agents. It includes a discussion of aspects of US and European Union law concerning risky activities, and then develops the economic analyses that are relevant to implementing choices within a supply and demand framework. The book also discusses exposure-response and time-series models used in assessing air and water pollution, as well as probabilistic cancer models, including toxicological compartmental, pharmaco-kinetic models and epidemiological relative risks and odds ratios-based models. Statistical methods to measure agreement, correlation and discordance are also developed. The methods and criteria of decision-analysis, including several measures of value of information (VOI) conclude the expositions. This book is an excellent text for students studying risk assessment and management.
With oil spills occurring worldwide, much media and practical attention has been given in recent years to the rapidly maturing field of hydrocarbon bioremediation, particularly with application to marine spills. Hydrocarbon contamination of soil and groundwater, although less visible, is even more widespread and has provided the background for the numerous studies presented in this book, in addition to those devoted to shoreline spills. Chapters address a wide variety of theory and practice and cover important subjects such as biofiltration, natural attenuation, surfactants, and the use of in situ bioventing compared to soil venting. This unique book represents the collective global experience of practitioners and researchers in North America, Europe, Africa, and Asia. It describes experiences in tying laboratory studies to field applications. Nowhere else can anyone involved in hydrocarbon bioremediation find more up-to-date, relevant information on field experience using the various techniques and combinations of techniques in remediating hydrocarbons by biological means.
The Leviathan Factor tells the incredible story of how Satan, created as Lucifer the morning star, self-transformed into Leviathan, God's serpentine arch foe. When he tried to achieve immortality by tweaking creation's lowest-level laws (a sophisticated computer/automaton) he created death instead. As the serpent he reappeared in the Genesis tree of good and evil, where he seduced humans to attempt immortality apart from covenant with God. Leviathan is responsible for the false belief that we each have an inner divine spark which, when reconnected to our ego, awakens our true inherent divinity. Unfortunately he and his demonic spirits also impact our minds, bodies, and environment as psi. A ...