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The Protein Protocols Handbook
The Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. All chapters are written in the same format as that used in the Methods in Molecular BiologyTM series. Each chapter opens with a description of the basic theory behind the method being described. The Materials section lists all the chemicals, reagents, buffers, and other materials necessary for carrying out the protocol. Since the principal goal of the book is to p...
The Proteomics Protocols Handbook
Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. All laboratory-based protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cardiology at the Limits
The first symposium in 1997 was dedicated to the initiation of the Chair of Cellular Cardiology, a joint venture between the University of Cape Town and University College London. This sixth title of symposia tests and re-tests the limits of our cardiological knowledge.
Visual Culture
This book is about the expanding realm of visual culture: in architecture, art, design, advertising, photography, film, television, video, theatre performance, computer imagery and virtual reality. It is also about Visual Culture Studies, a relatively new academic discipline, or rather range of disciplines, that scholars employ to analyse visual artefacts. Unlike many other texts on the same subject, it foregrounds the ‘visual’ and is systematic and accessible. Visual culture provides an overview of the subject that pays heed to the achievements of both traditional and new theory whilst directing the reader to a large body of literature via references and an extensive bibliography. Walker and Chaplin discuss the concepts of ‘the visual’ and of ‘culture’ as well as the field and origins of Visual Culture Studies; coping with theory; models of production and consumption; institutions; pleasure; the canon and concepts of value; visual literacy and poetics; modes of analysis; culture and commerce; and new technologies. This book is designed for those studying the history and theory of fine arts, design and the mass media.
Medical BioMethods Handbook
John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are southern and western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy.
Walker, R.N.
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Final Supplement to the Environmental Impact Statement for an Amendment to the Pacific Northwest Regional Guide: Appendices
Siskiyou County Library has vol. 1 only.
