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GTPases Regulating Membrane Dynamics
Provides a comprehensive set of articles describing the use and application of state-of-the-art methodologies to identify and characterize these GTPases and their expanding list of regulators and effectors. This work also includes methodologies focused on biochemical, molecular and advanced imaging techniques.
Glycobiology
In the past decade, there has been an explosion of progress in understanding the roles of carbohydrates in biological systems. This explosive progress was made with the efforts in determining the roles of carbohydrates in immunology, neurobiology and many other disciplines, examining each unique system and employing new technology. This volume represents the first of three in the Methods in Enzymology series, including Glycomics (vol. 416) and Functional Glycomics (vol. 417), dedicated to disseminating information on methods in determining the biological roles of carbohydrates. These books are designed to provide an introduction of new methods to a large variety of readers who would like to participate in and contribute to the advancement of glycobiology. The methods covered include structural analysis of carbohydrates, biological and chemical synthesis of carbohydrates, expression and determination of ligands for carbohydrate-binding proteins, gene expression profiling including micro array, and generation of gene knockout mice and their phenotype analyses.
Biological Mass Spectrometry
Describes and integrates the techniques of many advances in both chromatographic and mass spectrometric technologies. This book also covers various biophysical applications, such as H/D exchange for study of conformations, protein-protein and protein-metal and ligand interactions. It also describes atto-to-zepto-mole quantitation of 14C and 3H.
Macromolecular Crystallography
Annotation Accurate molecular structures is vital for rational drug design and for structure based functional studies directed toward the development of effective therapeutic agents and drugs. Crystallography can reliably predict structure, both in terms of folding and atomic details of bonding. * Phases * Map interpretation and refinement * Analysis and software.
Numerical Computer Methods, Part E
The contributions in this volume emphasize analysis of experimental data and analytical biochemistry, with examples taken from biochemistry. They serve to inform biomedical researchers of the modern data analysis methods that have developed concomitantly with computer hardware.Selected Contents: A practical approach to interpretation of SVD results; modeling of oscillations in endocrine networks with feedback; quantifying asynchronous breathing; sample entropy; wavelet modeling and processing of nasal airflow traces
Regulators of G Protein Signaling, Part B
Regulators of G Protein Signaling, Part B continues the in-depth treatment of the topic and covers the RSG protein superfamily including RZ, R4, R7, R12, RhoGEF, and GRK, as well as other heterotrimeric G-protein signaling regulators. - Table of Contents - RZ Subfamily - R4 Subfamily - R7 Subfamily - R12 Subfamily - RhoGEF Subfamily - GRK Subfamily - Other RGS proteins - Activators - Inhibitors - Other Modulators
Protein Engineering
This MIE volume covers methods for a multitude of topics among which are computational methods, laboratory methods, enzyme optimization, binding proteins/antibodies, and screening technologies.Table of Contents-Methodology-Applications-Opzimization and Screening-Applications-Directed Evolution of Enzymatic Function-Applications-Evolution of Biosynthetic Pathways-Devices, Antibodies and Vaccines
Adult Stem Cells
This is the second of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques focusing on adult stem cells, and written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a "cookbook and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using "conventional and novel methods, such as derivation of ES cells from sing...
Two-Component Signaling Systems, Part A
Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system." Sections include: Computational Analyses of Sequences and Sequence Alignments Biochemical and Genetic Assays of Individual Components of Signaling Systems Physiological Assays and Readouts - Presents detailed protocols - Includes troubleshooting tips
Molecular Evolution, Producing the Biochemical Data, Part B
The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences. Molecular Evolution Producing the Biochemical Data part B is a continuation of methods published in Part A (1993, volume 224). The work is a very methodological look at markers, templates, genomes, datasets and analyses used in studies of biological diversity.* One of the most highly respected publications in the field of biochemistry since 1955 * Frequently consulted, and praised by researchers and reviewers alike * Truly an essential publication for anyone in any field of the life sciences
