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Research on Nitrification and Related Processes, Part A
State-of-the-art update on methods and protocols dealing with the detection, isolation and characterization of macromolecules and their hosting organisms that facilitate nitrification and related processes in the nitrogen cycle as well as the challenges of doing so in very diverse environments. - Provides state-of-the-art update on methods and protocols - Deals with the detection, isolation and characterization of macromolecules and their hosting organisms - Deals with the challenges of very diverse environments
Computer Methods Part B
The combination of faster, more advanced computers and more quantitatively oriented biomedical researchers has recently yielded new and more precise methods for the analysis of biomedical data. These better analyses have enhanced the conclusions that can be drawn from biomedical data, and they have changed the way that experiments are designed and performed. This volume, along with previous and forthcoming Computer Methods volumes for the Methods in Enzymology serial, aims to inform biomedical researchers about recent applications of modern data analysis and simulation methods as applied to biomedical research. - Presents step-by-step computer methods and discusses the techniques in detail to enable their implementation in solving a wide range of problems - Informs biomedical researchers of the modern data analysis methods that have developed alongside computer hardware - Presents methods at the "nuts and bolts" level to identify and resolve a problem and analyze what the results mean
Nanomedicine
This volume in the Methods in Enzymology series comprehensively covers Cancer, Cardiovascular and the central nervous system of Nanomedicine. With an international board of authors, this volume is split into sections that cover subjects such as Diabetes and nanotechnology as potential therapy, Nanomedicines for inflammatory diseases, and Development and use of ceramide nanoliposomes in cancer. - Comprehensively covers cancer and the cardiovascular and central nervous systems of nanomedicine - An international board of authors - Split into sections that cover subjects such as diabetes and nanotechnology as potential therapy, nanomedicines for inflammatory diseases, and the development and use of ceramide nanoliposomes in cancer
Guide to Techniques in Mouse Development, Part A
Guide to Techniques in Mouse Development, Part A comprehensively covers new technologies and methodologies that have appeared for the study of mouse development. - Update of volume 225 of Methods in Enzymology, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993 - Covers new technologies and methodologies, including: - new techniques for the cryopreservation of gametes and embryos - production of transgenic and null (knockout) animals (use of ES cells) - generation of conditional/inducible mutant animals - use of gene-trap mutagenesis - analysis of allele-specific expresion - use of new reporter constructs - humanizing of transgenic animals - transcript profiling of mouse development - imaging of mouse development - rederivation of animals and use of mouse genomics
Synthetic Biology
Synthetic biology encompasses a variety of different approaches, methodologies and disciplines and many different definitions exist. This volume covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA assembly and manipulation.
Integrins
An integrin, or integrin receptor, is an integral membrane protein in the plasma membrane of cells. It plays a role in the attachment of a cell to the extracellular matrix (ECM) and to other cells, and in signal transduction from the ECM to the cell. There are many types of integrin, and many cells have multiple types on their surface. Integrins are of vital importance to all metazoans, from humans to sponges. This volume in Methods in Enzymology presents methods for studying integrins.
Methods in Enzymology
Since the inception of the series, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all field of life sciences. This final volume in the five-part Nitric Oxide series supplements MIE volumes 268, 269, 301 and 359. Nitric Oxide impinges on a wide range of fields in biological research, particularly in the areas of biomedicine and cell and organic biology, as well as fundamental chemistry. These volumes are a valuable resource for the experienced researcher and for those entering the field. *One of the most highly respected publication in the field of biochemistry since 1955 *Frequently consulted and praised by researchers and reviewers alike *Truly an essential publication for anyone in any field of the life sciences
Cellulases
Cellulase refers to a class of enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis. This volume of Methods in Enzymology comprehensively covers this topic. With an international board of authors, this volume covers subjects such as "The DNSA reducing assay for measuring cellulases," "Measuring processivity" and "In situ cellulose detection with carbohydrate-binding modules." - Comprehensively covers the topic of cellulases - International board of authors
Fragment Based Drug Design
There are numerous excellent reviews on fragment-based drug discovery (FBDD), but there are to date no hand-holding guides or protocols with which one can embark on this orthogonal approach to complement traditional high throughput screening methodologies. This Methods in Enzymology volume offers the tools, practical approaches, and hit-to-lead examples on how to conduct FBDD screens. The chapters in this volume cover methods that have proven to be successful in generating leads from fragments, including chapters on how to apply computational techniques, nuclear magnetic resonance, surface plasma resonance, thermal shift and binding assays, protein crystallography, and medicinal chemistry in...
Methods in Methane Metabolism
Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This volume focuses on microbial methane metabolism, which is central to the global carbon cycle. Both methanotrophy and methanogenesis are covered in detail. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state of the art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements The state-of-the-art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field
