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Protein Fluorescence
  • Language: en
  • Pages: 320

Protein Fluorescence

The intrinsic or natural fluorescence of proteins is perhaps the most complex area of biochemical fluorescence. Fortunately the fluorescent amino acids, phenylalanine, tyrosine and tryptophan are relatively rare in proteins. Tr- tophan is the dominant intrinsic fluorophore and is present at about one mole % in protein. As a result most proteins contain several tryptophan residues and even more tyrosine residues. The emission of each residue is affected by several excited state processes including spectral relaxation, proton loss for tyrosine, rotational motions and the presence of nearby quenching groups on the protein. Additionally, the tyrosine and tryptophan residues can interact with eac...

Principles of Fluorescence Spectroscopy
  • Language: en
  • Pages: 961

Principles of Fluorescence Spectroscopy

The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.

Advanced Concepts in Fluorescence Sensing
  • Language: en
  • Pages: 333

Advanced Concepts in Fluorescence Sensing

Over the last decade, fluorescence has become the dominant tool in biotechnology and medical imaging. These exciting advances have been underpinned by the advances in time-resolved techniques and instrumentation, probe design, chemical / biochemical sensing, coupled with our furthered knowledge in biology. Complementary volumes 9 and 10, Advanced Concepts of Fluorescence Sensing: Small Molecule Sensing and Advanced Concepts of Fluorescence Sensing: Macromolecular Sensing, aim to summarize the current state of the art in fluorescent sensing. For this reason, Drs. Geddes and Lakowicz have invited chapters, encompassing a broad range of fluorescence sensing techniques. Some chapters deal with small molecule sensors, such as for anions, cations, and CO2, while others summarize recent advances in protein-based and macromolecular sensors. The Editors have, however, not included DNA or RNA based sensing in this volume, as this were reviewed in Volume 7 and is to be the subject of a more detailed volume in the near future.

Topics in Fluorescence Spectroscopy
  • Language: en
  • Pages: 524

Topics in Fluorescence Spectroscopy

Time-resolved fluorescence spectroscopy is widely used as a research tool in bioch- istry and biophysics. These uses of fluorescence have resulted in extensive knowledge of the structure and dynamics of biological macromolecules. This information has been gained by studies of phenomena that affect the excited state, such as the local environment, quenching processes, and energy transfer. Topics in Fluorescence Spectroscopy, Volume 4: Probe Design and Chemical Sensing reflects a new trend, which is the use of time-resolved fluorescence in analytical and clinical chemistry. These emerging applications of time-resolved fluorescence are the result of continued advances in laser detector and computer technology. For instance, pho- multiplier tubes (PMT) were previously bulky devices. Miniature PMTs are now available, and the performance of simpler detectors is continually improving. There is also considerable effort to develop fluorophores that can be excited with the red/ne- infrared (NIR) output of laser diodes. Using such probes, one can readily imagine small time-resolved fluorometers, even hand-held devices, being used fordoctor’s office or home health care.

Nano-Optics for Enhancing Light-Matter Interactions on a Molecular Scale
  • Language: en
  • Pages: 470

Nano-Optics for Enhancing Light-Matter Interactions on a Molecular Scale

  • Type: Book
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  • Published: 2012-12-04
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  • Publisher: Springer

This volume presents a considerable number of interrelated contributions dealing with the new scientific ability to shape and control matter and electromagnetic fields on a sub-wavelength scale. The topics range from the fundamental ones, such as photonic metamateriials, plasmonics and sub-wavelength resolution to the more applicative, such as detection of single molecules, tomography on a micro-chip, fluorescence spectroscopy of biological systems, coherent control of biomolecules, biosensing of single proteins, terahertz spectroscopy of nanoparticles, rare earth ion-doped nanoparticles, random lasing, and nanocoax array architecture. The various subjects bridge over the disciplines of physics, biology and chemistry, making this volume of interest to people working in these fields. The emphasis is on the principles behind each technique and on examining the full potential of each technique. The contributions that appear in this volume were presented at a NATO Advanced Study Institute that was held in Erice, Italy, 3-18 July, 2011. The pedagogical aspect of the Institute is reflected in the topics presented in this volume.

Reviews in Plasmonics 2017
  • Language: en
  • Pages: 328

Reviews in Plasmonics 2017

Reviews in Plasmonics is a comprehensive collection of current trends and emerging hot topics in the field of Plasmonics and closely related disciplines. It summarizes the years progress in Plasmonics and its applications, with authoritative analytical reviews specialized enough to be attractive to professional researchers, yet also appealing to the wider audience of scientists in related disciplines of Plasmonics.

Peptide Synthesis and Applications
  • Language: en
  • Pages: 263

Peptide Synthesis and Applications

Hands-on experts describe in step-by-step detail the key methodologies of contemporary peptide synthesis and illustrate their numerous applications. The techniques presented include protocols for chemical ligation, the synthesis of cyclic and phosphotyrosine-containing peptides, lipoamino acid- and sugar-conjugated peptides, and peptide purification and analyses. Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases. A practical guide to the identification of proteins using mass spectrometric analyses of peptide mixtures is also included.

Radiative Decay Engineering
  • Language: en
  • Pages: 476

Radiative Decay Engineering

During recent years our enthusiasm for this field has continually increased. This book presents expert contributions describing the fundamental principles for the widespread use of radiative decay engineering in the biological sciences and nanotechnology.

Introduction to Fluorescence
  • Language: en
  • Pages: 316

Introduction to Fluorescence

The phenomenon known as fluorescence is now widely used in the chemical and life sciences largely due to the development of highly sophisticated fluorescent probe chemistries and the commercial availability of these probes as well as the development of novel microscopy approaches. Introduction to Fluorescence helps readers acquire a sound understanding of basic fluorescence theory and practice. It describes general principles in a straightforward way and uses examples from a variety of disciplines to demonstrate them. In color throughout, the book takes readers through the history of important discoveries to the most current advances. It introduces the fundamentals of the fluorescence phenom...

Biophysical and Biochemical Aspects of Fluorescence Spectroscopy
  • Language: en
  • Pages: 305

Biophysical and Biochemical Aspects of Fluorescence Spectroscopy

Fluorescence spectroscopy has traditionally found wide application in bio chemistry and cell biology. Since there are relatively few naturally occurring fluorescent biomolecules, fluorescence spectroscopy offers a combination of great specificity and sensitivity. Historically, these features have been ex ploited with great success utilizing both intrinsic and extrinsic probes. Re cent applications have built upon these traditional strengths and have re sulted in the development of new instrumental techniques, novel and convenient fluorescent probes, and a deeper, theoretical understanding of fundamental processes. Frequently, fluorescence techniques are tailored to attack a specific biologic...