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The past decade has seen the field of proteomics expand from a highly technical endeavor to a widely utilized technique. The objective of this book is to highlight the ways in which proteomics is currently being employed to address issues in the biological sciences. Although there have been significant advances in techniques involving the utilization of proteomics in biology, fundamental approaches involving basic sample visualization and protein identification still represent the principle techniques used by the vast majority of researchers to solve problems in biology. The work presented in this book extends from overviews of proteomics in specific biological subject areas to novel studies that have employed a proteomics-based approach. Collectively they demonstrate the power of established and developing proteomic techniques to characterize complex biological systems.
The field of proteomics has advanced considerably over the past two decades. The ability to delve deeper into an organism’s proteome, identify an array of post-translational modifications and profile differentially abundant proteins has greatly expanded the utilization of proteomics. Improvements to instrumentation in conjunction with the development of these reproducible workflows have driven the adoption and application of this technology by a wider research community. However, the full potential of proteomics is far from being fully exploited in plant biology and its translational application needs to be further developed. In 2011, a group of plant proteomic researchers established the ...
This book presents evidence-based approaches and techniques used to diagnose and manage organic solutes, oxidative stress, and antioxidant enzymes in crop plants under abiotic stressors. It discusses strategies in abiotic stress tolerance including osmoregulation, osmoprotectants, and the regulation of compatible solutes and antioxidant enzymes in plants. With contributions from 49 scholars worldwide, this authoritative guide is educational for scientists working with plants and abiotic stressors. Provides comprehensive coverage of all aspects of abiotic stress, from abiotic stresses’ effects on plant growth, development, and defense mechanisms, to functionality of enzymatic and non-enzyma...
The proteome comprises all protein species resulting from geneexpression in a cell, organelle, tissue or organism. By definition,proteomics aims to identify and characterise the expressionpattern, cellular location, activity, regulation,post-translational modifications, molecular interactions, threedimensional structures and functions of each protein in abiological system. In plant science, the number of proteome studies is rapidlyexpanding after the completion of the Arabidopsis thaliana genomesequence, and proteome analyses of other important or emergingmodel systems and crop plants are in progress or are beinginitiated. Proteome analysis in plants is subject to the sameobstacles and limit...
Mitochondrial biogenesis is an extremely complex process. A hint of this complexity is clearly indicated by the many steps and factors required to assemble the respiratory complexes involved in oxidative phosphorylation. These steps include the expression of genes present in both the nucleus and the organelle, intricate post-transcriptional RNA processing events, the coordinated synthesis, transport and assembly of the different subunits, the synthesis and assembly of co-factors and, finally, the formation of supercomplexes or respirasomes. It can be envisaged, and current knowledge supports this view, that plants have evolved specific mechanisms for the biogenesis of respiratory complexes. ...
The use of nanomaterials for remediation efforts has been overlooked even though they present interesting characteristics as remediators. This new book provides a valuable overview of low-cost and eco-friendly green synthesized nanomaterials as advantageous technology that promotes efficient nanoremediation of environmental pollution for the restoration of polluted areas. It discusses the potential of nanomaterials, specifically green synthesized nanomaterials, as a practical and efficient solution toward sustainability. The book details the advantages of green nanomaterials when compared to conventional physicochemical methods, such as avoiding the use of harmful reagents and reducing toxic waste production. The book addresses themes such as contaminants associated with environmental pollution and the threats to humans; nanoremediation strategies that use microbes, plants, or amendments; and nanoparticles as tools for nanoremediation and their advantageous characteristics.
The conversion of lignocellulosic biomass into renewable fuels and other commodities has provided an appealing alternative towards supplanting global dependence on fossil fuels. The suitability of multitudes of plants for deconstruction to useful precursor molecules and products is currently being evaluated. These studies have probed a variety of phenotypic traits, including cellulose, non-cellulosic polysaccharide, lignin, and lignin monomer composition, glucose and xylose production following enzymatic hydrolysis, and an assessment of lignin-carbohydrate and lignin-lignin linkages, to name a few. These quintessential traits can provide an assessment of biomass recalcitrance, enabling resea...