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Ancient DNA refers to DNA which can be recovered and analyzed from clinical, museum, archaeological and paleontological specimens. Ancient DNA ranges in age from less than 100 years to tens of millions of years. The study of ancient DNA is a young field, but it has been revolutionized by the application of polymerase chain reaction technology, and interest is growing very rapidly. Fields as diverse as evolution, anthropology, medicine, agriculture, and even law enforcement have quickly found applications in the recovery of ancient DNA. This book contains contributions from many of the "first generation" researchers who pioneered the development and application of ancient DNA methods. Their c...
New techniques and updated protocols for the detection and analysis of biomolecules - proteins, glycoproteins and nucleic acids. The second edition of this successful laboratory manual describes in detail the highly sensitive systems which are widely used in molecular biological and biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody/antigen binding or hybridisation as well as PCR amplification. The clearly structured step-by-step protocols with practical hints and a troubleshooting guide are complemented by chapters on the theoretical background and the application of the techniques, enabling scientists to plan, design and conduct the appropriate procedures.
This volume of conference papers presents new discoveries, updated information, and technological advances in the study of the Dead Sea Scrolls. Two papers examine the identity of the messiahs in 4Q246 and 4Q521. A thorough analysis of scribal markings in the Dead Sea texts is presented. Biblical studies include multiple literary editions of biblical texts, the book of Numbers at Qumran, and the appearance of the Tetragrammaton in 4QSama texts. The notions of judgment and salvation according to Sapiential Work A are thoroughly examined, and the relationship of the six Barki Nafshi texts is carefully considered. New developments in the field of Dead Sea Scrolls studies include the Dead Sea Scrolls Database and DNA studies on the scrolls themselves.
This manual describes the methodology and applications of microinjection, a powerful technique for transposing material into living cells through needle injection. Information is presented on the techniques principles, the required equipment, preparation of receiving cells and injected material, and the analysis of results. Detailed protocols for a wide range of applications in different cellular systems are included. A large part of the book is also dedicated to the Xenopus laevis oocyte system.
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This manual surveys toxins from insects, spiders, mollusks, fish, and snakes which have biotechnological applications. It reviews aspects of toxin origin, their molecular mechanism, and their cellular and pathogenic effects. It also provides methodology for the application of these toxins in the research laboratory. This includes a description of the extraction methods, biochemical characterization, and applications in pharmacological studies.
Contributors detail up-to-date guidelines for using molecular techniques, cytogenetic and linkage analysis, and cellular methods, emphasizing human cells and medically relevant research. They present results of recent applications of techniques and step-by-step protocols for cloning large DNA molecu
Prokaryotic Genomics provides molecular microbiologists in particular and researchers working with bacteria in general with the most important established recipes needed for their work. The volume covers both revisited classical methods and new tools for global analysis such as genomics or proteomics. It is written for those in need of a bench manual to complete their experiments and for those wanting to understand the modern tools used in microbiology.
"As someone who has spent forty years in psychology with a long-standing interest in evolution, I'll just assimilate Howard Bloom's accomplishment and my amazement."-DAVID SMILLIE, Visiting Professor of Zoology, Duke University In this extraordinary follow-up to the critically acclaimed The Lucifer Principle, Howard Bloom-one of today's preeminent thinkers-offers us a bold rewrite of the evolutionary saga. He shows how plants and animals (including humans) have evolved together as components of a worldwide learning machine. He describes the network of life on Earth as one that is, in fact, a "complex adaptive system," a global brain in which each of us plays a sometimes conscious, sometimes ...
On December 6, 1961, a contract was signed by which the research institute of the Wander AG in Freiburg became the Max-Planck-Institut fUr Immunbiologie. The transfer of ownership took place during a happy expansion phase of the Max-Planck Society in which a growing economy in Germany allowed the foundation of many new research institutes by the Max-Planck-Society and other organizations. Nevertheless, it was a remarkable event. The acquisition by an academic organization of an institute formerly operated by an industrial company was rather unusual, not to speak ofthe fact that not only the facilities but also the entire scientific personnel were taken over. Retrospectively, the 40 years of ...