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Completely revised to reflect the innovations in HPLC from the past decade, this authoritative reference presents practical strategies for the evaluation and analysis of proteins, peptides, and polynucleotides. Offering class-specific applications for the characterization and fractionation of biological macromolecules, the book contains material on organic supports, size exclusion, ion exchange, hydrophobic interaction, and metal interaction chromatography. Leading experts summarize specialized detection systems, provides discussions on the chemical and biological properties of specific biomolecules, include detailed guidelines for the development of analytical techniques, and more.
A convenient source of information for workers in analytical chemistry, experimental biology, physics, and engineering, this Second Edition stands as a quick reference source and clear guide to specific chromatographic techniques and principles-providing a basic introduction to the science and technology of the method, as well as additional references on the theory and methodology for analysis of specific chemicals and applications in a range of industries.
Column Handbook for Size Exclusion Chromatography is the first comprehensive reference to provide everything one needs to know about commercial analytical and preparative columns for size exclusion and gel filtration chromatography (SEC and GFC). SEC is now widely used as a quality assurance method in the polymer industry (both synthetic and biopolymers) to determine molecular weight and molecular weight distribution. The Handbook contains contributions from every column manufacturer around the world and from many experienced column users. It covers the technology, characterization, application, evaluation, maintenance, and quality control of analytical and preparative columns for SEC and GFC. Also included are columns for two closely related techniques, hydrodynamic chromatography and high osmotic pressure chromatography.Key Features* Evaluate and select columns with confidence for specific applications* Optimize separations and improve the ruggedness of analytical methods* Extend the service time of a column* Establish a quality-control program to ensure consistency in column performance* Avoid the expense of column damage or purchases that do not give the expected results
This volume comprehensively relates developments, principles, and applications of combined liquid chromatography-mass spectrometry and other techniques such as capillary electrophoresis and supercritical fluid chromatography combined with mass spectrometry. It covers historical developments, currently important interfaces and technologies, and LC-MS applications in environmental analysis, pharmaceuticals and bioanalysis, and additional fields. It offers in-depth coverage of interfaces and technologies currently important in the laboratory, especially electrospray and APCI, contains an expanded applications section, and provides over 2200 references, tables, equations, and drawings.
Widely employed for separating and detecting chemicals in solution, separation techniques are most often applied in tandem, subsequently referred to as hyphenated methods. Hyphenated and Alternative Methods of Detection in Chromatography details the development and application of mass spectral detection techniques coupled with gas phase and liquid phase chromatographies. With contributions from experts in a variety of fields, as evidenced by the range of topics, the book describes the advantages and disadvantages of various separation techniques and addresses methods for cutting-edge applications such as proteomics research. Specifically, this book covers: The development of the LC-NMR and i...
This book provides a detailed description of technical elements of a microbore column liquid chromatograph suitable for use in trace analysis. It presents examples of analyses, especially from the spheres of biochemistry, pharmacology, and environmental analytical chemistry.
Berezkin (petrochemical synthesis, USSR Academy of Sciences) explains to chromatographers that gas-liquid chromatography, an exciting development of gas chromatography, is itself just a limited case of gas-liquid-solid chromatography, and shows how this perspective can help solve problems more quick
Covering the principles of chromatographic separation, the chromatographic process from a physical chemical perspective, instrumentation for performing analyses, and operational procedures, this second edition offers information needed for the successful practice of gas chromatography. It contains examples of available apparatus, detectors, columns, stationary phases and operating conditions.
This book summarizes all the important aspects of multidimensional separations, providing information on gas, liquid and thin-layer chromatography, as well as the techniques and applications of supercritical fluid chromatography in the multidimensional mode.
Considers three fundamental aspects of molecular interactions important in chromatography, taking care not to duplicate information readily available in other references. Surveys the basic factors involved in complex formation, which governs the retention mechanism and selectivity in either donor or