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When Frederick Watts announces that the family will be leaving England to homestead in Canada, seven-year-old Amy thinks it sounds like a grand adventure; however, even the boat ride doesn't go the way that she imagined it. As she nurses her seasick mother and siblings while her father drinks in the ship's smoking room, she begins to realize that her new life is not going to be easy. The Watts family faces not only the elements but also their personal demons as they struggle to create a new life in a strange land. As their lives become increasingly fraught with tragedy, they must draw on one another for strength. These circumstances soon force Amy and her siblings to shoulder responsibilitie...
Tumor Immunology and Immunotherapy – Molecular Methods, Volume 629, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this release include Droplet digital PCR for measuring circulating tumor-derived DNA, Detection and quantification of cytosolic DNA, Methods to detect endogenous dsRNA induction and recognition, Quantification of eIF2alpha phosphorylation during immunogenic cell death, Assessment of annexin A1 release during immunogenic cell death, Luciferase-assisted detection of extracellular ATP in the course of ICD, The P2X7 receptor: structure and function, and much more. - Contains the authority of authors who are leaders in their field - Provides a comprehensive source on new methods and research in enzymology
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Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. - Indispensable tool for the researcher - Carefully written and edited by experts to contain step-by-step protocols - In this volume we have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate
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Her vacation seems doomed until she finds Patti, one of her students recently snatched from the school grounds by her non-custodial father. When she discovers that Patti is very ill, she convinces the child's father to take her to the hospital. Once back at home, Kathleen becomes very involved in the lives of Patti and her family. Her boyfriend, Edward Connors, doesn't agree with her involvement, an involvement that eventually leads to a rift-possibly unmendable. When she runs into an old friend from high school, gossip in the small timber town spreads faster than chalk dust from beaten erasers, and touches the teacher. Will Kathleen, with God's help, know she's strong enough to stand on her own, withstand pressure from family and friends, and make up her own mind about her future? Will the Chalk Dust settle and Choices become clear?