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Analysis and improvement of the in vitro transfection efficiency of plasmid-DNA encoding for equine IL-12 used for melanoma therapy in horses
In horses studies targeting equine melanoma therapy using DNA encoding for cytokines as interleukin 12 (IL-12) have shown some promising results. Nevertheless, complete remission after treatment has been only observed in few cases, possibly due to reduced therapy efficiency. Herein transfection efficiency and methodology-induced cytotoxicity were analysed after transfection with different nanoparticle-mediated and conventional approaches using eukaryotic DNA-expression-plasmids and mammalian cell lines. The addition of gold nanoparticles (AuNPs) to the transfection protocols significantly increased transfection efficiency when compared to a conventional FHD mediated transfection protocol, an...
Science Breakthroughs to Advance Food and Agricultural Research by 2030
For nearly a century, scientific advances have fueled progress in U.S. agriculture to enable American producers to deliver safe and abundant food domestically and provide a trade surplus in bulk and high-value agricultural commodities and foods. Today, the U.S. food and agricultural enterprise faces formidable challenges that will test its long-term sustainability, competitiveness, and resilience. On its current path, future productivity in the U.S. agricultural system is likely to come with trade-offs. The success of agriculture is tied to natural systems, and these systems are showing signs of stress, even more so with the change in climate. More than a third of the food produced is uncons...
The Conservation Atlas of Tropical Forests
This final volume in the The Conservation Atlas of Tropical Forests covers the Americas. It provides an up-to-date overview of the status of rain forests in South America, Central America, and the Caribbean. Following the format of the two previous volumes The Conservation Atlas of Tropical Forests: Asia and the Pacific (1991) and The Conservation Atlas of Tropical Forests: Africa (1992), the atlas is divided into two parts. Part I introduces and discusses the complex interrelated issues in the regions that are involved in both deforestation as well as conservation of the tropical forests. Included are discussions on the history of the forests, agricultural colonization policies and deforestation, conservation polices for plants and wildlife, protected areas, and the future of the tropical forests. Part II is a detailed and well referenced country-by-country analysis of conservation status and trends. Four-colour maps have been compiled from satellite and radar imagery, aerial photography, and the latest information provided by forestry departments and development agencies.
F. Scott Fitzgerald in Context
Explores many of the important social, historical and cultural contexts surrounding the life and works of F. Scott Fitzgerald.
Tietz Clinical Guide to Laboratory Tests - E-Book
This new edition of Norbert Tietz's classic handbook presents information on common tests as well as rare and highly specialized tests and procedures - including a summary of the utility and merit of each test. Biological variables that may affect test results are discussed, and a focus is placed on reference ranges, diagnostic information, clinical interpretation of laboratory data, interferences, and specimen types. New and updated content has been added in all areas, with over 100 new tests added. - Tests are divided into 8 main sections and arranged alphabetically. - Each test includes necessary information such as test name (or disorder) and method, specimens and special requirements, r...
Handbook of Developmental Disabilities
This authoritative handbook reviews the breadth of current knowledge about developmental disabilities: neuroscientific and genetic foundations; the impact on health, learning, and behavior; and effective educational and clinical practices. Leading authorities analyze what works in intervening with diverse children and families, from infancy through the school years and the transition to adulthood. Chapters present established and emerging approaches to promoting communication and language abilities, academic skills, positive social relationships, and vocational and independent living skills. Current practices in positive behavior support are discussed, as are strategies for supporting family adaptation and resilience.
The Data Science Design Manual
This engaging and clearly written textbook/reference provides a must-have introduction to the rapidly emerging interdisciplinary field of data science. It focuses on the principles fundamental to becoming a good data scientist and the key skills needed to build systems for collecting, analyzing, and interpreting data. The Data Science Design Manual is a source of practical insights that highlights what really matters in analyzing data, and provides an intuitive understanding of how these core concepts can be used. The book does not emphasize any particular programming language or suite of data-analysis tools, focusing instead on high-level discussion of important design principles. This easy...
Decolonizing Pathways towards Integrative Healing in Social Work
Taking a new and innovative angle on social work, this book seeks to remedy the lack of holistic perspectives currently used in Western social work practice by exploring Indigenous and other culturally diverse understandings and experiences of healing. This book examines six core areas of healing through a holistic lens that is grounded in a decolonizing perspective. Situating integrative healing within social work education and theory, the book takes an interdisciplinary approach, drawing from social memory and historical trauma, contemplative traditions, storytelling, healing literatures, integrative health, and the traditional environmental knowledge of Indigenous Peoples. In exploring issues of water, creative expression, movement, contemplation, animals, and the natural world in relation to social work practice, the book will appeal to all scholars, practitioners, and community members interested in decolonization and Indigenous studies.
The Elephant in the Room
ONE OF NPR’S BEST BOOKS OF 2019 A “warm and funny and honest…genuinely unputdownable” (Curtis Sittenfeld) memoir chronicling what it’s like to live in today’s world as a fat man, from acclaimed journalist Tommy Tomlinson, who, as he neared the age of fifty, weighed 460 pounds and decided he had to change his life. When he was almost fifty years old, Tommy Tomlinson weighed an astonishing—and dangerous—460 pounds, at risk for heart disease, diabetes, and stroke, unable to climb a flight of stairs without having to catch his breath, or travel on an airplane without buying two seats. Raised in a family that loved food, he had been aware of the problem for years, seeing doctors a...
Billboard
In its 114th year, Billboard remains the world's premier weekly music publication and a diverse digital, events, brand, content and data licensing platform. Billboard publishes the most trusted charts and offers unrivaled reporting about the latest music, video, gaming, media, digital and mobile entertainment issues and trends.
