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Culture of Human Stem Cells
This book collects the most effective and cutting-edge methods and protocols for deriving and culturing human embryonic and adult stem cells—in one handy resource. This groundbreaking book follows the tradition of previous books in the Culture of Specialized Cells Series—each methods and protocols chapter is laid out exactly like the next, with stepwise protocols, preceded by specific requirements for that protocol, and a concise discussion of methods illustrated by data. The editors describe a limited number of representative techniques across a wide spectrum of stem cells from embryonic, newborn, and adult tissue, yielding an all-encompassing and versatile guide to the field of stem cell biology and culture. The book includes a comprehensive list of suppliers for all equipment used in the protocols presented, with websites available in an appendix. Additionally, there is a chapter on quality control, and other chapters covering legal and ethical issues, cryopreservation, and feeder layer culture. This text is a one-stop resource for all researchers, clinical scientists, teachers, and students involved in this crucial area of study.
Culture of Epithelial Cells
"...a wonderful compendium of current in vitro approaches that will be a useful resource to those just starting to work with an epithelial cell system as well as those that have been working with them for years and years." --Pharmaceutical Research This completely revised and expanded new edition provides detailed descriptions of fundamental and practical aspects relating to the in vitro cultivation of disparate types of epithelia. In recent years, the use of epithelial cell culture in cell biology and tissue engineering has increased dramatically. This revision reflects those advances by including new chapters on the culture of animal and human hepatocytes, kidney epithelium, and bladder epithelium. Each chapter provides an introductory review of the principles and advantages of the particular method, followed by detailed protocols, practical tips, alternate methods, and a useful list of materials and suppliers.
Culture of Cells for Tissue Engineering
Step-by-step, practical guidance for the acquisition, manipulation,and use of cell sources for tissue engineering Tissue engineering is a multidisciplinary field incorporatingthe principles of biology, chemistry, engineering, and medicine tocreate biological substitutes of native tissues for scientificresearch or clinical use. Specific applications of this technologyinclude studies of tissue development and function, investigatingdrug response, and tissue repair and replacement. This area israpidly becoming one of the most promising treatment options forpatients suffering from tissue failure. Written by leading experts in the field, Culture of Cellsfor Tissue Engineering offers step-by-step,...
Chemokine Protocols
The chemokines family of small proteins are involved in numerous b- logical processes ranging from hematopoiesis, angiogenesis, and basal l- kocyte trafficking to the extravasation and tissue infiltration of leukocytes in response to inflammatory agents, tissue damage, and bacterial or viral infection. Chemokines exert their effects through a family of seven G-protein coupled transmembrane receptors. Worldwide interest in the chemokine field surged dramatically early in 1996, with the finding that certain chemokine receptors were the elusive coreceptors, required along with CD4, for HIV infection. Today, though over 40 human chemokines have been described, the n- ber of chemokine receptors l...
Culture of Immortalized Cells
This is a detailed, yet concise manual which provides the most up-to-date in vitro methods & safety procedures for the immortalization of primary cultures for various cell types. Each method is accompanied by step by step protocols & instructions.
Freshney's Culture of Animal Cells
FRESHNEY’S CULTURE OF ANIMAL CELLS THE NEW EDITION OF THE LEADING TEXT ON THE BASIC METHODOLOGY OF CELL CULTURE, FULLY UPDATED TO REFLECT NEW APPLICATIONS INCLUDING IPSCS, CRISPR, AND ORGAN-ON-CHIP TECHNOLOGIES Freshney’s Culture of Animal Cells is the most comprehensive and up-to-date resource on the principles, techniques, equipment, and applications in the field of cell and tissue culture. Explaining both how to do tissue culture and why a technique is done in a particular way, this classic text covers the biology of cultured cells, how to select media and substrates, regulatory requirements, laboratory protocols, aseptic technique, experimental manipulation of animal cells, and much ...
Culture of Human Tumor Cells
Ein neuer Band aus der 'Culture of Specialized Cells'-Reihe. Leserfreundlich aufgemacht. Er vermittelt spezifische praktische Details, wie man Medien und Reagenzien sowie Protokolle für Zellisolierung und Zellkultur präpariert. Logisch aufgebaut und nach spezifischen Tumoren gegliedert. Farbtafeln demonstrieren anschaulich Immunozytochemie und Fluoreszenz in situ Hybridisierung (FISH). Darüber hinaus beschreibt das Buch auch umfangreiche Sicherheitsvorkehrungen. Mit einer Vielzahl nützlicher Tipps. Mit einem Glossar zu ausgewählten Fachtermini. Enthält eine umfangreiche Liste mit Bezugsadressen von Ausrüstung und Zellkulturprodukten. Erläutert medikamentöse Behandlung, Auswahl, Differenzierung, Assays für die Untersuchung maligner Zellen sowie Risiken und Anwendungsmöglichkeiten.
DNA Transfer to Cultured Cells
Der DNA Transfer in Zellen erlaubt entweder das Einbringen von neuen Genen in Zellen oder die Überexpression von Genen, die bereits existieren, jedoch auf niedrigem Niveau ausgeprägt sind. Die rasanten Fortschritte in der DNA Transfertechnologie hat Veränderungen in vielen Disziplinen nach sich gezogen, von der Molekulargenetik bis hin zur Biotechnolgie. (10/98) Dieses Buch ist das erste Nachschlagewerk, das Informationen zu bahnbrechenden Techniken für den Transfer von Genmaterial in Zellen (in-vitro) liefert. Von führenden Wissenschaftlern geschrieben, behandelt dieses Handbuch eine Vielzahl von Transfermethoden und deren Anwendungsmöglichkeiten bei speziellen Zelltypen sowie Aspekte der Molekularbiologie bei Gewebekulturen. Auf unnötige technische Einzelheiten wird verzichtet, wodurch die Behandlung der Techniken und Themenbereiche überschaubar bleibt. Dieser handliche und benutzerfreundliche Band spricht nicht nur die wichtigsten Entwicklungen der letzten Jahre an, sondern ebenso die aktuellen Trends beim DNA Transfer.
Culture of Hematopoietic Cells
This guide provides a comprehensive synthesis of proven methods for the culture and experimental manipulation of haemopoietic cells. Covering a range of human and mouse cell types, and featuring step-by-step laboratory procedures, it gives detailed instructions for reagent preparation.
