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The first comprehensive treatise on Rapid Cycle Real-Time PCR. With amplification times of 15-30 minutes of on-line detection and analysis, nucleic acid quantification of mutation analysis finally becomes a routine, powerful and rapid method. Focusing primarily on the LightCycler, an instrument that combines Rapid Cycle PCR with fluorescent monitoring, this technology provides convenient analysis by melting temperatures. PCR products can be identified by product Tm, and single base mismatches can easily be genotyped by probe Tm. Methods chapters detail the theory behind quantification of mutation analysis; the design of synthesis of fluorescent hybridization probes of the preparation of template DNA. Application chapters apply nucleid acid quantification to infectious organisms of intracellular messengers and mutation detection to somatic of acquired mutations.
The preanalytical phase is an important component of Laboratory medicine and errors arising in this phase affect the validity of laboratory results. In this book physicians and clinical staff have access to valuable information about the current preanalytical variables and factors (patient preparation, sample collection, handling and processing before analysis).
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In times of global economic and political crises, the notion of solidarity is gaining new currency. This book argues that a solidarity-based perspective can help us to find new ways to address pressing problems. Exemplified by three case studies from the field of biomedicine: databases for health and disease research, personalised healthcare, and organ donation, it explores how solidarity can make a difference in how we frame problems, and in the policy solutions that we can offer.
Ein Gesamtüberblick – evidenzbasiert und leitlinienorientiert: Neueste Therapiestrategien und Behandlungsempfehlungen für den klinischen Alltag. Umfassende diagnostische Möglichkeiten. Anschauliche Grafiken zu Diagnostik und Behandlung. Mit wertvollen Praxistipps. Aktuelle wissenschaftliche Grundlagen: Gliederung in die Themenfelder: Grundlagen, Diagnostik und Therapie, klinische Diabetologie, Diabetes und Gesundheitswesen. Berücksichtigung sozial- und arbeitsrechtlicher Aspekte sowie psychologischer und gesundheitspolitischer Gesichtspunkte. Lösungskonzepte für die Diabetestherapie: Die Bedeutung der digitalen Transformation und Diabetestechnologie für die Therapie des Diabetes. Or...