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Microarray Technology Through Applications provides the reader with an understanding, from an applications perspective, of the diverse range of concepts required to master the experimental and data analysis aspects of microarray technology. The first chapter is a concise introduction to the technology and provides the theoretical background required to understand the subsequent sections. The following chapters are a series of case studies representative of the most general and important applications of microarray technology, including CGH, analysis of gene expression, SNP arrays and protein arrays. The case studies are written by experts in the field and describe prototypic projects, indicating how to generalize the approach to similar studies. There are detailed step-by-step protocols describing the specific experimental and data analysis protocols mentioned in the case study section. There is also information on printing glass DNA microarray slides and data interpretation. Colour figures and data sets are provided on the website at http://www.garlandscience.com/9780415378536
The sheer volume of prolific Spanish novelist and playwright Benito Pérez Galdós's literary production has rendered overall assessment of his body of work all but impossible. The later volumes in his ambitious and popular Episodios nacionales series, in particular, have suffered from scholarly indifference. In this acclaimed study, Brian J. Dendle closely considers the twenty-six novels in this series written between 1898 and 1912. These episodios, Dendle contests, are artistically superior to the earlier volumes and offer a unique opportunity to establish the ideological profile of the mature Galdós.
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Spain and the Great Powers in the Twentieth Centuryexamines the international context to, and influences on, Spanish history and politics from 1898 to the present day. Spanish history is necessarily international, with the significance of Spain's neutrality in the First World War and the global influences on the outcome of the Spanish Civil War. Taking the Defeat in the Spanish American war of 1898 as a starting point, the book includes surveys on: *the crisis of neutrality during the First World War *foreign policy under the dictatorship of Primo de Rivera *the allies and the Spanish Civil War *Nazi Germany and Franco's Spain *Spain and the Cold War *relations with the United States This book traces the important topic of modern Spanish diplomacy up to the present day
Physical desire and metaphysical love in the theatre of Federico García Lorca. A dialectical tension between physical desire and metaphysical love lies at the heart of the theatre works of Federico García Lorca, and the deployment of queer theory's critique of gender and identity is surprisingly effective inthis discussion of love versus desire. Seldom is enough attention paid to the poet's early works, and so this book offers a timely review of the 'religious tragedy' Cristo, as well as Mariana Pineda, uncoveringin these early offerings an explicit proposal of the supremacy of love over desire. A meditation on the fragmentary and challenging El público yields a vivid panorama of identity in crisis, and a paradigmatic Lorcan sacrifice of self for love. The ostensibly more conventional tragedies of Amor de Don Perlimplín con Belisa en su jardín and Yerma are also reassessed in terms of self-sacrifice and self-love. The study concludes with an argument for a practical re-reading of La casa de Bernarda Alba, which emphasises how the play might be saved from po-faced realism with music, humour and drag performance. PAUL McDERMID lectures in Spanish at Queen's University Belfast.
This is an account of Spain's disastrous war with the United States in 1898, in which she lost the remnants of her old empire. The book also analyzes the ensuing political and social crisis in Spain from the loss of empire, through World War I, to the military coup of 1923.
Based on extensive archival research and containing rare and previously unpublished photos, this book provides the most detailed reconstruction ever of one of the most important events in Spanish theatrical history.Winner of the 2019-20 AHGBI-Spanish Embassy Publication Prize On 18 June 1933, one of the most important events in Spanish theatrical history took place before an audience of 3,000 spectators in the ruins of the Roman Theatre in Mérida. Translated into Spanish by philosopher Miguel de Unamuno, staged by the renowned Xirgu-Borràs Company and funded by the government, the performance of Seneca''s Medea was a triumph of republican culture and widely hailed for its new dramatic and ...
The "idle fictions" of the vanguard novel of the 1920s and 1930s in Spain and Spanish America represented a kind of interlude of playfulness--a vacation or parenthetical insertion--in what was perceived as the established course of the modern Hispanic novel's development. Yet, as Pérez Firmat argues, though this genre saw itself as recreative and interstitial, it deliberately precipitated "a class war not between social classes but between literary classes." Concentrating on source material not widely available, Pérez Firmat reconstructs the reception these novels received at the time of their publication, then develops a reading of them based on the intellectual context of this reception. A new preface and an appendix on vanguard biographies have been added to this paperback edition.
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Molecular biology has rapidly advanced since the discovery of the basic flow of information in life, from DNA to RNA to proteins. While there are several important and interesting exceptions to this general flow of information, the importance of these biological macromolecules in dictating the phenotypic nature of living creatures in health and disease is paramount. In the last one and a half decades, and particularly after the completion of the Human Genome Project, there has been an explosion of technologies that allow the broad characterization of these macromolecules in physiology, and the perturbations to these macromolecules that occur in diseases such as cancer. In this volume, we wil...