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Little Goat wanders off to look for the sweet grass and doesn’t realise how far she is from Mother Goat. Story Attribution: ‘Little Goat’ is written by Mirna Lawrence. © Book Dash, 2016. Some rights reserved. Released under CC BY 4.0 license. (http://creativecommons. org/licenses/by/4.0/) Other Credits: This story 'Little Goat' has been published on StoryWeaver by BookDash.
Under the aegis of the post-apartheid government, much emphasis has been placed on the transformation and democratisation of the heritage sector in South Africa since 1994. The emergent new landscape of memory relies heavily on commemorative monuments, memorials and statues aimed at reconciliation, nation-building and the creation of a shared public history. But not everyone identifies with these new symbolic markers and their associated interpretation of the past. Drawing on a number of theoretical perspectives, this book critically investigates the flourishing monument phenomenon in South Africa, the political discourses that fuel it; its impact on identity formation, its potential benefits, and most importantly its ambivalences and contradictions.
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MicroRNAs (miRNAs), endogenous noncoding regulatory mRNAs of - nucleotides, have rapidly emerged as the central players in gene expression regulation. Owing to their ever-increasing implications in the control of various biological and pathological processes, miRNAs have now been considered novel biomarkers of various human diseases including, cancer, viral disease, cardiov- cular disorders, metabolic disturbances, etc. Particular expression pro?les have been associated with particular pathological states. Expression pro?ling of miR- NAs have therefore become extremely important not only for fundamentalists but also for clinicians. However, the methodologies used for detecting protein-coding...
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This second edition provides updated and comprehensive methods on miRNA biogenesis and their role in the development and progression of various human diseases. Chapters detail miRNA biogenesis, isolating RNA, extracellular vesicles (EVs), circulating miRNAs, analyzing miRNA and miRDeep-P2, protocols for total RNA isolation from cells, cell-derived products, isolation and characterization of exosomes, serum, plasma specimens, and software tools. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, MicroRNA Profiling: Methods and Protocols, Second Edition aims to provide comprehensive and accessible methods to undergraduate, graduate, and established scientist.
MicroRNAs (miRNAs), endogenous noncoding regulatory mRNAs of around 22-nucleotides long, have rapidly emerged as one of the key governors of the gene expression regulatory program in cells of varying species, with ever-increasing implications in the control of the fundamental biological processes and in the pathogenesis of adult humans. The exciting findings in this field have inspired us with a premise and a promise that miRNAs will ultimately be taken to the heart for therapy of human disease. While miRNAs have been considered potential therapeutic targets for disease treatment, it remains obscured what strategies we can use to achieve the goal. In the past years, we have witnessed a rapid...