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A collection of cutting-edge techniques for using capillary electrophoresis (CE) to analyze complex carbohydrates. These readily reproducible protocols provide methods for sample preparation, analysis of mono- and oligosaccharides, glycoproteins, and glycoconjugates. A useful appendix describes the structures of the most commonly encountered carbohydrate residues and olgosaccharides from mammalian and bacterial origins. Each protocol contains detailed information on reagents, apparatus, notes, comments, and tips on procedures.
The analysis and modification of glycans of recombinant proteins continues to be active and challenging area of research and for the successful manufacture of these proteins. In Cell Engineering, volume 3: Glycosylation, Dr. Mohammed Al-Rubeai has compiled a group of articles that will provide research workers not only with reviews of the advances that have been made in all facets of the subject but with an in-depth assessment of the state of the art methodology and the various approaches for the improvement of glycoprotein production. Particularly important in this respect is the advances made in the development of genetically engineered host cell lines with novel glycosylation properties, as well as the integration of mass spectrophotometric analysis with separation techniques. This volume is intended not only for research students and senior scientists in cell culture and glycobiology, but also for industrial biotechnologists and biochemical engineers interested in the production of therapeutic glycoproteins, virus vector and ex vivo expansion of human cells for medical treatment.
This book is an updated and expanded edition of Carbohydrate Analysis, High Performance Liquid Chromatography and Capillary Electrophoresis and is concerned with the analysis of carbohydrates by modern chromatography and electrophoresis including analytical and preparative high performance liquid chromatography (HPLC), thin layer chromatography (TLC), field flow fractionation (FFF), capillary electrophoresis (CE), capillary electrochromatography (CEC), polyacrylamide gel electrophoresis (PAGE), gas chromatography (GC) and supercritical fluid chromatography (SFC). Thirty-one chapters cover: various modes of HPLC, CE, CEC, FFF, GC and SFC that are currently applied to the analysis of carbohydr...
Simple carbohydrates, complex oligosaccharides and polysaccharides all belong to a class of ubiquitous (macro)molecules that exhibit a wide range of biological functions, and the recent advent of enhanced enzymatic, chemical and analytical tools used to study these sugars has inaugurated a genuine explosion in the field of glycomics. Specifically, it has led to a deeper understanding of how specific sugar structures modulate cellular phenotypes, and that breakthrough has led to the discovery of new pharmaceuticals for the treatment of many serious diseases, such as cancer. The subsequent rapid expansion of this research holds high promise for future therapeutic regimens, and capillary electr...
Dr. Abdelhamid Elaissari, internationally respected author and researcher, reports on and analyzes a broad range of important findings from new studies on the use of colloidal nanoparticles in biomedical, food, and environmental diagnostics and analyses. Throughout the presentation, the book uses a blend of classical tools, including optical microscopy, atomic force microscopy, microsystems, and microfluidics, to help you take full advantage of colloidal nanoparticles for your own research and applications.
Capillary electrophoresis (CE) has become an established method with widespread recognition as an analytical technique of choice in numerous analytical laboratories, including industrial and academic sectors. Pharmaceutical and biochemical research and quality control are the most important CE applications. This book provides a comparative assessment of related techniques on mode selection, method development, detection, and quantitative analysis and estimation of pharmacokinetic parameters and broadens the understanding of modern CE applications, developments, and prospects. It introduces the fundamentals of CE and clearly outlines the procedures used to mitigate several barriers, such as detection limits, signal detection, changing capillary environment, resolution separation of analytes, and hyphenation of mass spectrometry with CE, for a range of analytical problems. Each chapter outlines a specific electrophoretic variant with detailed instructions and some standard operating procedures. In this respect, the book meets its desired goal of rendering assistance to lovers of electrophoresis.
For more than five decades, scientists and researchers have relied on the Advances in Chromatography series for the most up-todate information on a wide range of developments in chromatographic methods and applications. The clear presentation of topics and vivid illustrations for which this series has become known makes the material accessible and engaging to analytical, biochemical, organic, polymer, and pharmaceutical chemists at all levels of technical skill. Key Features: Includes a chapter dedicated to Izaak Maurits Kolthoff, offering a unique look at his non-professional life as well as his impact and legacy in Analytical Chemistry. Discusses recent advances in two-dimensional liquid chromatography for the characterization of monoclonal antibodies and other therapeutic proteins. Reviews solvation processes, methodologies of their measurement, and parameters influenced solvation Explores recent advances in TLC analysis of natural colorings, determination of synthetic dyes, and determination of EU-permitted natural colors, in foods. Offers comprehensive and critical insights on the key aspects of CE-MS analysis of intact proteins