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Plant Genotyping: From Traditional Markers to Modern Technologies
In contrast to the external traits of plants, we cannot directly see the genotypes that comprise the underlying set of genetic material encoding these phenotypic traits. To make genotypes accessible for research and further understanding, various genotyping methods are used. Plant genotyping began with relatively simple and elementary molecular markers, like microsatellites or SSR (Simple Sequence Repeats), which were then followed by DNA sequencing and fragment analysis, PCR and qPCR, allele-specific molecular probes and primers, and now today’s modern and advanced microchip-DNA technology involving hundreds and thousands of reactions simultaneously.
Plant Genotyping
This thorough volume presents a wide range of existing methods, from the very popular to the more exotic, in the area of plant genotyping. Many methods of plant genotyping were initially developed for medical research, but all genotyping methods, if they are to be successful, should be suitable for application across the full range of studies within plant biology, as seen in this collection. Plant genotyping methods herein are based on a variety of assessments, including DNA microarray, with its hundreds of thousands of simultaneous reactions, or separate individual studies of DNA sequencing and fragment analysis, PCR and qPCR, allele-specific molecular probes and primers, digestion with res...
Genome
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Ome-wide Studies of Grapevine Fruit Composition and Responses to Agro-environmental Factors in the Era of Systems Biology
Fruits play a substantial role in the human diet as a source of vitamins, minerals, dietary fiber and a wide range of molecules relevant to health promotion and disease prevention. The characterization of genes involved in the accumulation of these molecules during fruit development and ripening, and in the overall plant’s response to the environment, constitutes a fundamental step for improving yield- and quality-related traits, and for predicting this crop’s behavior in the field. This is certainly the case for grapevine (Vitis vinifera L.), one of the most largely cultivated fruit crops in the world. The cultivation of this species is facing challenging scenarios driven by climate cha...
