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A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation. - Describes techniques for detection of [Ca2+]I: Ca2+ - sensitive microelectrodes - Fluorescent dyes - Luminescent proteins - Includes techniques for perturbing intracellular Ca2+ - Covers detailed methodology plus problems and pitfalls of each technique - Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program - Color plates illustrate techniques such as - Confocal ratio-imaging - Use of aequorin
This volume is a comprehensive guide to the methodologies used in the study of structural domains of cell nuclei. The text covers chromatin, the karyoskeleton, the soluble domain, and the nucleolus. It details methods that are used to isolate components from these domains and techniques used to assemble and disassemble nuclear elements. There is also coverage of three-dimensional mapping and localization of nuclear processes. Key Features * Provides a practical laboratory guide for studying cell nuclei * Includes comprehensive and easy-to-follow protocols
This new volume in the Current Topics in Developmental Biology series concentrates on transcriptional switches during development. It includes chapters on such topics as muscle diversity, transcriptional response to genome structural variation, and the temporal gene network in Drosophila neural stem cells. With an international team of authors, this volume is a must-have addition for researchers and students alike. - Concentrates on transcriptional switches during development - Includes chapters on such topics as muscle diversity, transcriptional response to genome structural variation, and the temporal gene network in Drosophila neural stem cells - With an international team of authors, this volume is a must-have addition for researchers and students alike
A comprehensive look at the state of the art in detection technologies and materials used in the development of diagnostics for clinical, medicinal and environmental applications.
Every cell of the body is dependent on calcium to function. Calcium is found in teeth and bones, and calcium signalling is necessary for the movement of muscles and for the action of the heart and the intestines as well as blood coagulation. Calcium in Living Cells will update classic techniques in detecting microscopic levels of calcium ions (Ca2+) in living cells, as well as address new techniques in the field of calcium detection and calcium signaling. Such detection and measurement of intracellular calcium is important to researchers studying the heart, musculoskeletal, gastrointestinal, and immune systems, whose findings will aid in the advancement of drug and genomic therapies to treat...
Together with other volumes in this series, Volume 58 presents thoughtful and forward-looking articles on developmental biology and developmental medicine. Reviews include:* A role for endogenous electric fields in wound healing* The role of mitotic checkpoint in maintaining genomic stability * The regulation of oocyte maturation* Stem cells: A promising source of pancreatic islets for transplantation in type 1 diabetes* Differentiation potential of adipose derived adult stem (ASAS) cellsThe exceptional reviews in this volume of Current Topics in Developmental Biology will be valuable to both clinical and fundamental researchers, as well as students and other professionals who want an introduction to current topics in cellular and molecular approaches to developmental biology and clinical problems of aberrant development.* Series Editor Gerald Schatten is one of the leading minds in reproductive and developmental science* Presents major issues and astonishing discoveries at the forefront of modern developmental biology and developmental medicine* The longest-running forum for contemporary issues in developmental biology with over 30 years of coverage
Bioengineering and Biophysical Aspects of Electromagnetic Fields primarily contains discussions on the physics, engineering, and chemical aspects of electromagnetic (EM) fields at both the molecular level and larger scales, and investigates their interactions with biological systems. The first volume of the bestselling and newly updated Handbook of Biological Effects of Electromagnetic Fields, Third Edition, this book adds material describing recent theoretical developments, as well as new data on material properties and interactions with weak and strong static magnetic fields. Newly separated and expanded chapters describe the external and internal electromagnetic environments of organisms and recent developments in the use of RF fields for imaging. Bioengineering and Biophysical Aspects of Electromagnetic Fields provides an accessible overview of the current understanding on the scientific underpinnings of these interactions, as well as a partial introduction to experiments on the interactions themselves.
Published in affiliation with the Society for Developmental Biology.
Cilia are highly conserved organelles that serve motile functions, sensory functions, or both. These organelles power cell movement, generate fluid flow in various organs, act as sensors of the extracellular environment and have been modified for various specialized tasks such as light reception and smell. Defects in these ubiquitous organelles lead to a broad array of human genetic disorders that range from polycystic kidney disease, retinal degeneration, epilepsy and infertility to developmental defects such as situs inversus and polydactyly. This volume is the third in a three-part series on cilia that focuses on the use of model organisms to gain insight into ciliary function and on the process of intraflagellar transport that is essential for the assembly and maintenance of ciliary structures. - Includes both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time - Covers forward and reverse genetic analysis of IFT and biochemical methods to define the role of IFT components - Methods presented cover molecular, genetic, and biochemical approaches to ciliary function in model organisms