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Under the vast umbrella of Plant Sciences resides a plethora of highly specialized fields. Botanists, agronomists, horticulturists, geneticists, and physiologists each employ a different approach to the study of plants and each for a different end goal. Yet all will find themselves in the laboratory engaging in what can broadly be termed biotechnol
Biotechnology revolutionized traditional plant breeding programs. This rapid change produced new discussions on techniques and opportunities for commerce, as well as a fear of the unknown. Plant Development and Biotechnology addresses the major issues of the field, with chapters on broad topics written by specialists. The book applies an informal style that addresses the major aspects of development and biotechnology with minimal references, without sacrificing information or accuracy. Divided into five primary parts, this volume explores how the field emerged from its early theoretical base to the technical discipline of today. It also covers progress being made with genetically engineered ...
Instructors, students and researchers in plant pathology have been searching for a primary text that combines an informal, easy-to-read style with a thorough introduction to the concepts and terminology of plant pathology. Plant Pathology Concepts and Laboratory Exercises answers their demand by presenting pathology principles, protocols and procedures, serving as a valuable resource tool for both students and researchers. This guide explains definitions of disease, characteristics of organisms that cause disease, and how diseases interact with hosts and the environment. Each topic is addressed by an expert in the field, and is supported by one or more lab exercises. The structure of the text allows for easy reading, with references minimized and major concepts highlighted at the beginning of each chapter. The laboratory exercises give added flexibility to instructors. There are experiments for both beginning and advanced students, and a broad choice of exercise topics that can be selected based upon the focus within each individual class. Step-by-step instructions are provided for each laboratory exercise.
Includes a DVD Containing All Figures and Supplemental Images in PowerPointThis new edition of Plant Propagation Concepts and Laboratory Exercises presents a robust view of modern plant propagation practices such as vegetable grafting and micropropagation. Along with foundation knowledge in anatomy and plant physiology, the book takes a look into t
Alternating between topic discussions and hands-on laboratory experiments that range from the in vitro flowering of roses to tissue culture of ferns, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, addresses the most current principles and methods in plant tissue culture research. The editors use the expertise of some of the top researchers and educators in plant biotechnology to furnish students, instructors and researchers with a broad consideration of the field. Divided into eight major parts, the text covers everything from the history of plant tissue culture and basic methods to propagation techniques, crop improvement procedures, specialized applications and nut...
Introduction and techniques; Introductory history; Laboratory organisation; Media; Aseptic manipulation; Basic aspects; Cell culture; Cellular totipotency; Somatic embryogenesis; Applications to plant breeding; Haploid prodution; Triploid production; In vitro pollination and fertilization; Zygotic embryo culture; Somatic hybridisation and cybridisation; Genetic transformation; Somaclonal and gametoclonal variant selection; Application to horticulture and forestry; Production of disease-free plants; clonal propagation; General applications; Industrial applications: secondary metabolite production; Germplasm conservation.
Microsatellites or simple sequence repeats (SSRs) have become the markers of choice for a variety of molecular studies because of their versatility, operational flexibility, and lower cost than other marker systems. Microsatellites: Methods and Protocols brings together experts in the field to cover this significant area of research. Broken in to four convenient parts, this volume delves into classical and modern methods for the discovery and development of microsatellite markers, descriptions of amplification and visualization of SSRs, automated capillary sequencers that are widely used for fragment analysis, as well as a variety of methods for the analysis of data obtained by the use of mi...
PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.
Agroforestry in Sustainable Agricultural Systems examines the environmental and social conditions that affect the roles and performance of trees in field- and forest-based agricultural production systems. Various types of ecological settings for agroforestry are analyzed within temperate and tropical regions. The roles of soil, water, light, nutrient and pest management in mixed, annual, woody perennial and livestock systems are discussed. Important new case studies from around the world offer innovative strategies that have been used successfully in raising forests and tree products on a sustainable basis for commercial harvesting and for providing other environmental services in land conservation and watershed management.
Plant Pathology presents information and advances in plant pathology including disease induction and development and disease resistance and control. This book is organized into two major parts encompassing 14 chapters that focus on diseases, pathogenicity, and pathogen variability. The first part of the book deals with general considerations of disease, the disease cycle, parasitism and pathogenicity, and the variability in pathogens. This is followed by a presentation of the mechanisms by which pathogens cause disease and plants resist disease. Core chapters focus on the effects of pathogen-produced enzymes, toxins, growth regulators, and polysaccharides on the structural organization and o...