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Metagenomics
This volume provides up-to-date and novel techniques for various screening technologies currently used in metagenomics and related areas. Starting with DNA/RNA isolation from environmental samples, the book continues by delving into areas such as current methods used to isolate DNA and construct metagenomic libraries, establishment of metagenome libraries in non-E. coli hosts, and topics like function-driven mining of metagenomic DNA, screening and analyzing protocols for a wide array of different genes encoding enzymes, bacterial viruses and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Metagenomics: Methods and Protocols, Third Edition provides a comprehensive collection of up-to-date metagenome protocols and tools for the recovery of many major types of biocatalysts and allows for the easy setup of these screens in microbiology laboratories.
Molecular Ecology and Genetic Diversity of the Roseobacter Clade
Marine bacteria and archaea are key players in the biogeochemical cycling of nitrogen, carbon, and other elements. One important lineage of marine bacteria is the Roseobacter group. Members of this clade are the most abundant bacteria in marine ecosystems constituting up to 25% of the marine bacterioplankton. They have been detected in various marine habitats from coastal regions to deep-sea sediments and from polar regions to tropical latitudes. These bacteria are physiologically and genetically very versatile. Utilization of several organic and inorganic compounds, sulfur oxidation, aerobic anoxygenic photosynthesis, carbon monoxide oxidation, DMSP demethylation, and production of secondary metabolites are some of the important functional traits found in this clade. Moreover, several isolates are available allowing in-depth analysis of physiological and genetic characteristics. Although the Roseobacter group has been intensively studied in recent years, our understanding of its ecological contributions and the evolutionary processes shaping the genomes of this clade is still rather limited.
Handbook of Molecular Microbial Ecology II
The premiere two-volume reference on revelations from studying complex microbial communities in many distinct habitats Metagenomics is an emerging field that has changed the way microbiologists study microorganisms. It involves the genomic analysis of microorganisms by extraction and cloning of DNA from a group of microorganisms, or the direct use of the purified DNA or RNA for sequencing, which allows scientists to bypass the usual protocol of isolating and culturing individual microbial species. This method is now used in laboratories across the globe to study microorganism diversity and for isolating novel medical and industrial compounds. Handbook of Molecular Microbial Ecology is the fi...
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